In Immunopeptidomics We Need a Sniper Instead of a Shotgun

被引：62

作者：

Faridi, Pouya ^{[1
,2
]}

Purcell, Anthony W. ^{[1
,2
]}

Croft, Nathan Paul ^{[1
,2
]}

机构：

[1] Monash Univ, Infect & Immun Program, Biomed Discovery Inst, Clayton, Vic, Australia

[2] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic, Australia

来源：

PROTEOMICS | 2018年 / 18卷 / 12期

基金：

澳大利亚国家健康与医学研究理事会; 澳大利亚研究理事会; 英国医学研究理事会;

关键词：

fragmentation; immunopeptidomics; peptide identification; sequencing; MHC CLASS-I; ANTIGEN PRESENTATION; PROJECT; PEPTIDES; LIGANDS;

D O I：

10.1002/pmic.201700464

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Immunopeptidomics employs the use of mass spectrometry to identify and quantify peptides presented on the surface of cells by major histocompatibility complex (MHC; human leukocyte antigen [HLA], in humans) molecules, an essential component of adaptive immunity. Currently, immunopeptidomics follows the same or similar workflows as the more established field of shotgun proteomics, yet inherent differences between these two fields create significant drawbacks for the former. In this viewpoint, we would like to highlight such technical issues and provide suggestions for novel workflows that would increase peptide sequencing coverage, depth, and confidence, collectively enhancing the capabilities of the field of immunopeptidomics.

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页数：4

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