Optimization of eGFP expression using a modified baculovirus expression system

被引：11

作者：

Ge, Jingping ^{[1
]}

Jin, Liying ^{[1
]}

Tang, Xiaoyan ^{[1
]}

Gao, Dongni ^{[1
]}

An, Qi ^{[1
]}

Ping, Wenxiang ^{[1
]}

机构：

[1] Heilongjiang Univ, Coll Life Sci, Key Lab Microbiol, Harbin 150080, Peoples R China

来源：

JOURNAL OF BIOTECHNOLOGY | 2014年 / 173卷

基金：

中国国家自然科学基金;

关键词：

WSSV ie1 promoter; WPRE; ITRs; VSV-GED; Recombinant baculovirus; POSTTRANSCRIPTIONAL REGULATORY ELEMENT; INVERTED TERMINAL REPEATS; EFFICIENT GENE-TRANSFER; ADENOASSOCIATED VIRUS; TRANSGENE EXPRESSION; MAMMALIAN-CELLS; RECOMBINANT BACULOVIRUS; IN-VIVO; CATIONIC LIPOSOMES; AMPLICON VECTORS;

D O I：

10.1016/j.jbiotec.2014.01.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The baculovirus gene expression system is an efficient and safe protein expression system, since baculoviruses cannot replicate in mammalian cells. In order to improve the transduction efficiency and increase the reporter gene expression levels delivered by baculoviruses, we tested in the baculovirus expression cassette the Woodchuck hepatitis virus response element (WPRE), and AAV-derived inverted terminal repeats (ITRs) and the truncated vesicular stomatitis virus G protein (VSV-GED). The results showed that WPRE and VSV-GED have synergistic effects and could enhance the expression efficiency of enhanced green fluorescence protein (eGFP), and that ITRs effectively extended the duration of eGFP expression. We also demonstrated that the efficiency of eGFP expression varied under the control of the CMV, CBA, EF1-alpha or WSSV ie1 promoters in different cell lines. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：41 / 46

页数：6

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