Turnover rate and stimulus-evoked release of dopamine by progesterone and N-methyl-D-aspartic acid in rat striatum during pregnancy

The proposed modulatory role of progesterone on dopaminergic nerve terminal activity in the striatum was examined in pregnant rats. Endogenous dopamine concentration and the in vitro effect of exogenous progesterone in association with N-methyl-D-aspartic acid (NMDA) upon [H-3]dopamine release from striatal slices were determined. Striatal dopamine and 3,4-dihidroxyphenylacetic acid (Dopac) contents on day 5 of pregnancy were significantly higher than those found at the other stages of pregnancy and proestrus. On days 5 and 15 of pregnancy, progesterone (400 nM) was able to enhance [H-3]dopamine release stimulated by NMDA (50 mu M) A similar effect was found in striatal slices from proestrus rats. In contrast, progesterone was without an effect on days 1, 10 and 20 of pregnancy and postpartum. The results suggest that an increased synthesis and/or release of dopamine takes place on certain days of pregnancy and, simultaneously, that there is a significant increase in the responsiveness of striatal dopaminergic nerve terminals to excitatory inputs. They provide further support for a modulatory role of progesterone in relation with a glutamatergic action on dopaminergic activity in the corpus striatum.