A microfluidic cell co-culture platform with a liquid fluorocarbon separator

被引：18

作者：

Brewer, Bryson M. ^{[1
]}

Shi, Mingjian ^{[2
,3
]}

Edd, Jon F. ^{[1
]}

Webb, Donna J. ^{[2
,3
,4
]}

Li, Deyu ^{[1
]}

机构：

[1] Vanderbilt Univ, Dept Mech Engn, Nashville, TN 37235 USA

[2] Vanderbilt Univ, Dept Biol Sci, Nashville, TN 37235 USA

[3] Vanderbilt Univ, Vanderbilt Kennedy Ctr Res Human Dev, Nashville, TN 37235 USA

[4] Vanderbilt Univ, Dept Canc Biol, Nashville, TN 37235 USA

来源：

BIOMEDICAL MICRODEVICES | 2014年 / 16卷 / 02期

基金：

美国国家科学基金会;

关键词：

Cell culture; Cell co-culture; Synaptic formation; SOFT LITHOGRAPHY; CULTURE-SYSTEM; 2-PHASE FLOW; BUBBLE TRAP; POLY(DIMETHYLSILOXANE); DEVICES; MICROCHANNELS; STABILIZATION; HEPATOCYTES; MICROCHIPS;

D O I：

10.1007/s10544-014-9834-8

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A microfluidic cell co-culture platform that uses a liquid fluorocarbon oil barrier to separate cells into different culture chambers has been developed. Characterization indicates that the oil barrier could be effective for multiple days, and a maximum pressure difference between the oil barrier and aqueous media in the cell culture chamber could be as large as similar to 3.43 kPa before the oil barrier fails. Biological applications have been demonstrated with the separate transfection of two groups of primary hippocampal neurons with two different fluorescent proteins and subsequent observation of synaptic contacts between the neurons. In addition, the quality of the fluidic seal provided by the oil barrier is shown to be greater than that of an alternative solid-PDMS valve barrier design by testing the ability of each device to block low molecular weight CellTracker dyes used to stain cells in the culture chambers.

引用

页码：311 / 323

页数：13

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