Control of actin assembly and disassembly at filament ends

被引:266
作者
Cooper, JA [1 ]
Schafer, DA [1 ]
机构
[1] Washington Univ, Dept Cell Biol, St Louis, MO 63110 USA
关键词
D O I
10.1016/S0955-0674(99)00062-9
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The most important discovery in the field is that the Arp2/3 complex nucleates assembly of actin filaments with free barbed ends. Arp2/3 also binds the sides of actin filaments to create a branched network. Arp2/3's nucleation activity is stimulated by WASP family proteins, some of which mediate signaling from small G-proteins. Listeria movement caused by actin polymerization can be reconstituted in vitro using purified proteins: Arp2/3 complex, capping protein, actin depolymerizing factor/cofilin, and actin. actin depolymerizing factor/cofilin increases the rate at which actin subunits leave pointed ends, and capping protein caps barbed ends.
引用
收藏
页码:97 / 103
页数:7
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