Development of a polymerase chain reaction and restriction typing assay for the diagnosis of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections

被引：9

作者：

De-Giuli, L ^{[1
]}

Magnino, S ^{[1
]}

Vigo, PG ^{[1
]}

Labalestra, I ^{[1
]}

Fabbi, M ^{[1
]}

机构：

[1] Ist Zooprofilatt Sperimentale Lombardia & Emilia, Sez Diagnost Pavia, I-27100 Pavia, Italy

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2002年 / 14卷 / 04期

关键词：

D O I：

10.1177/104063870201400417

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become it powerful diagnostic tool.

引用

页码：353 / 356

页数：4

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