Development of a polymerase chain reaction and restriction typing assay for the diagnosis of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections

被引：9
|
作者：
De-Giuli, L [1 ]
Magnino, S [1 ]
Vigo, PG [1 ]
Labalestra, I [1 ]
Fabbi, M [1 ]
机构：
[1] Ist Zooprofilatt Sperimentale Lombardia & Emilia, Sez Diagnost Pavia, I-27100 Pavia, Italy
来源：
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2002年 / 14卷 / 04期
关键词：
D O I：
10.1177/104063870201400417
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become it powerful diagnostic tool.
引用
收藏
页码：353 / 356
页数：4
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