Molecular characterization of ribonucleotide reductase from Cryptosporidium parvum

被引:6
作者
Akiyoshi, DE [1 ]
Balakrishnan, R [1 ]
Huettinger, C [1 ]
Widmer, G [1 ]
Tzipori, S [1 ]
机构
[1] Tufts Univ, Sch Vet Med, Div Infect Dis, North Grafton, MA 01536 USA
来源
DNA SEQUENCE | 2002年 / 13卷 / 03期
关键词
Cryptosporidium parvum; ribonucleoticle reductase; gene cloning and sequencing;
D O I
10.1080/10425170290023419
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The Apicomplexan enteric parasite, Cryptosporidium, infects a broad range of mammals, birds, fish and reptiles. Cryptosporidium parvum, the principal causative agent of human cryptosporidiosis, has emerged as a major contributor to waterborne outbreaks of this disease. The absence of effective drugs against C. parvum has necessitated the search for new drug targets. One attractive class of target enzymes is ribonucleotide reductase, an essential enzyme required for the de novo synthesis of deoxyribonucleotides. We report the cloning and sequencing of the small and large subunits of ribonucleotide reductase from a C. parvum genotype 2 isolate, GCH1. Southern blot analysis showed that these subunits are encoded by single copy genes. Both subunits have been expressed as recombinant proteins in Escherichia coli.
引用
收藏
页码:167 / 172
页数:6
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