Measurement of light absorption and determination of the specific rate of light uptake in cultures of phototrophic microorganisms

被引:9
|
作者
Sanchez, O [1 ]
Mas, J [1 ]
机构
[1] AUTONOMOUS UNIV BARCELONA,DEPT GENET & MICROBIOL,E-08193 BARCELONA,SPAIN
关键词
D O I
10.1128/AEM.62.2.620-624.1996
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
This article describes a novel method for the measurement of light absorption by cultures of phototrophic microorganisms. The rate of light absorption is calculated as the difference between the rate of light output from a culture containing cells and the rate of light output from a culture containing only growth medium. The specific rate of light uptake is Calculated by dividing the rate of light absorption by the total biomass present in the culture. Application of the method to several case studies shows that light output from a culture varies widely depending on the absorption and scattering characteristics of the suspension.
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页码:620 / 624
页数:5
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