DNA binding studies of 2-tert-butylhydroquinone (TBHQ) food additive

被引：142

作者：

Kashanian, S. ^{[1
,2
]}

Dolatabadi, J. Ezzati Nazhad ^{[3
]}

机构：

[1] Razi Univ, Fac Sci, Dept Chem, SBRC, Kermanshah, Iran

[2] Razi Univ, Fac Sci, NNRC, Kermanshah, Iran

[3] Razi Univ, Fac Sci, Dept Biol, Kermanshah, Iran

来源：

FOOD CHEMISTRY | 2009年 / 116卷 / 03期

关键词：

DNA; 2-tert-Butylhydroquinone; Intercalation; TERT-BUTYLHYDROQUINONE; FLUORESCENCE; COMPLEX; METABOLITE;

D O I：

10.1016/j.foodchem.2009.03.027

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The interaction of native calf thymus DNA with 2-tert-butylhydroquinone (TBHQ) in 10 mM Tris-HCl aqueous solutions at neutral pH 7.4, has been investigated by spectro photometric, spectrofluorometric, voltammetric and viscosimetric techniques. It is found that TBHQ molecules could intercalate between base pairs of DNA as are evidenced by: hyperchromism in UV absorption band of DNA, sharp increase in specific viscosity of DNA and decrease in the fluorescence of TBHQ solutions in the presence of increasing amounts of DNA. Also peak current decrease, positive shift in the cyclic voltammetry (CV) and differential pulse voltammetry (DPV) are other evidences to indicate that, TBHQ is able to be intercalated in the DNA base pairs. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：743 / 747

页数：5

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