Quantitative analysis of amino acids in protein hydrolysates

17 kinds of amino acids are separated and the molar absorption coefficients of their derivatives are determined by high performance liquid chromatography using 9-fluorenylmethylchloroformate as the pre-column derivatising agent. The standard solution of amino acids is quantitatively analyzed by absolute analysis method and standard curves method respectively, the relative deviation of the two methods is < 5.0% except of histine. The simulation hydrolytic experiments of amino acids are conducted in two situations, it is found that the degradations of unstable amino acids in 110°C/24 h are less than those in 150°C/1.5 h. The amino acids' contents of bovine insulin hydrolysate in different conditions are measured quantitatively by absolute analysis method, and the relative recovery of the amino acids is nearly 100%.