Rapid and sensitive LC-MS method for pharmacokinetic study of vinorelbine in rats

被引：9

作者：

Cheng, Tiefeng ^{[2
]}

Si, Duanyun ^{[1
]}

Liu, Changxiao ^{[1
]}

机构：

[1] Tianjin Inst Pharmaceut Res, Tianjin State Key Lab Pharmacokinet & Pharmacodyn, Tianjin 300193, Peoples R China

[2] Henan Univ, Key Lab Special Funct Mat, Kaifeng 475004, Peoples R China

来源：

BIOMEDICAL CHROMATOGRAPHY | 2009年 / 23卷 / 09期

基金：

美国国家科学基金会;

关键词：

vinorelbine; LC-ESI-MS; rat plasma; pharmacokinetics; METABOLITES; DERIVATIVES;

D O I：

10.1002/bmc.1216

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and sensitive LC-electrospray ionization-MS method was developed for determining vinorelbine in rat plasma. A 100 mu L plasma sample was treated using a protein precipitation procedure and was chromatographed within 4 min using an Inertsil ODS-3 C-18 (2.1 x 50 mm, 5 mu m) column. The selected ion monitoring ions [M + H](+) were m/z 779 and m/z 811 for vinorelbine and vinblastine (internal standard), respectively. The method validation showed that the calibration curve for vinorelbine was linear over a concentration range of 1-1000 ng/mL with lower limit of quantification at I ng/mL. The method has been successfully applied to pharmacokinetics in rat plasma. Copyright (C) 2009 John Wiley & Sons, Ltd.

引用

页码：909 / 911

页数：3

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