A novel fluorescent aptasensor for the highly sensitive and selective detection of cardiac troponin I based on a graphene oxide platform

被引:63
作者
Liu, Dongkui [1 ,2 ]
Lu, Xing [2 ]
Yang, Yiwen [2 ]
Zhai, Yunyun [2 ]
Zhang, Jian [2 ]
Li, Lei [2 ]
机构
[1] Changzhou Univ, Sch Petrochem Engn, Changzhou 213016, Jiangsu, Peoples R China
[2] Jiaxing Univ, Coll Biol Chem Sci & Engn, 118 Jiahang Rd, Jiaxing 314001, Zhejiang, Peoples R China
基金
中国国家自然科学基金;
关键词
cTnI; Fluorescent aptasensor; Graphene oxide; LABEL-FREE DETECTION; GOLD NANOPARTICLES; QUANTUM DOTS; APTAMER; IMMUNOSENSOR; IMMUNOASSAY; ELECTRODE; MYOGLOBIN; STRATEGY; CHIP;
D O I
10.1007/s00216-018-1076-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Acute myocardial infarction (AMI) is one of the leading risks to global health. Thus, the rapid, accurate early diagnosis of AMI is highly critical. Human cardiac troponin I (cTnI) has been regarded as a golden biomarker for AMI due to its excellent selectivity. In this work, a novel fluorescent aptasensor based on a graphene oxide (GO) platform was developed for the highly sensitive and selective detection of cTnI. GO binds to the fluorescent anti-cTnI aptamer and quenches its fluorescence. In the presence of cTnI, the fluorescent anti-cTnI aptamer leaves the surface of GO, combines with cTnI because of the powerful affinity of the fluorescent anti-cTnI aptamer and cTnI, and then restores the fluorescence of the fluorescent anti-cTnI aptamer. Fluorescence-enhanced detection is highly sensitive and selective to cTnI. The method exhibited good analytical performance with a reasonable dynamic linearity at the concentration range of 0.10-6.0 ng/mL and a low detection limit of 0.07 ng/mL (S/N = 3). The fluorescent aptasensor also exhibited high selectivity toward cTnI compared with other interference proteins. The proposed method may be a potentially useful tool for cTnI determination in human serum.
引用
收藏
页码:4285 / 4291
页数:7
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