A wide range optical pH sensor for living cells using Au@Ag nanoparticles functionalized carbon nanotubes based on SERS signals

被引：27

作者：

Chen, Peng ^{[1
]}

Wang, Zhuyuan ^{[1
]}

Zong, Shenfei ^{[1
]}

Chen, Hui ^{[1
]}

Zhu, Dan ^{[1
]}

Zhong, Yuan ^{[1
]}

Cui, Yiping ^{[1
]}

机构：

[1] Southeast Univ, Adv Photon Ctr, Nanjing 210096, Jiangsu, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2014年 / 406卷 / 25期

关键词：

p-Aminothiophenol; SERS; Multi-wall carbon nanotubes; pH sensor; ENHANCED RAMAN-SCATTERING; INTRACELLULAR PH; FLUORESCENT PROTEIN; SINGLE-MOLECULE; ROUGH SURFACES; GOLD NANORODS; IN-VIVO; AMINOTHIOPHENOL; PHOTOCHEMISTRY; SPECTROSCOPY;

D O I：

10.1007/s00216-014-8064-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

p-Aminothiophenol (pATP) functionalized multi-walled carbon nanotubes (MWCNTs) have been demonstrated as an efficient pH sensor for living cells. The proposed sensor employs gold/silver core-shell nanoparticles (Au@Ag NPs) functionalized MWCNTs hybrid structure as the surface-enhanced Raman scattering (SERS) substrate and pATP molecules as the SERS reporters, which possess a pH-dependent SERS performance. By using MWCNTs as the substrate to be in a state of aggregation, the pH sensing range could be extended to pH 3.0 similar to 14.0, which is much wider than that using unaggregated Au@Ag NPs without MWCNTs. Furthermore, the pH-sensitive performance was well retained in living cells with a low cytotoxicity. The developed SERS-active MWCNTs-based nanocomposite is expected to be an efficient intracellular pH sensor for bio-applications.

引用

页码：6337 / 6346

页数：10

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