A model of periodontitis in the rat: effect of lipopolysaccharide on bone resorption, osteoclast activity, and local peptidergic innervation

被引：101

作者：

Dumitrescu, AL ^{[1
]}

Abd El-Aleem, S ^{[1
]}

Morales-Aza, B ^{[1
]}

Donaldson, LF ^{[1
]}

机构：

[1] Univ Bristol, Sch Med Sci, Dept Physiol, Bristol BS8 1TD, Avon, England

来源：

JOURNAL OF CLINICAL PERIODONTOLOGY | 2004年 / 31卷 / 08期

基金：

英国医学研究理事会;

关键词：

bone resorption; calcitonin gene-related peptide; inflammation; lipopolysaccharide; osteoclast activation; periodontitis;

D O I：

10.1111/j.1600-051X.2004.00528.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Objective: To establish and characterise a rat model of periodontitis that reiterates the features of human disease. Methods: Periodontal inflammation was induced by a single injection of 10 mug liposaccharide (LPS) (Salmonella typhimurium) in 1 mul saline into rat mandibular gingiva at the buccomesial aspect of the second molar. Animals were killed after 3, 7 and 10 days, mandibles dissected and sectioned for histological and immunocytochemical analysis. Results: LPS injection resulted in a significant gingival and periodontal inflammation with inflammatory infiltrate, apical migration of the junctional epithelium, interdental bone loss, and activation of osteoclasts at the site of injection 7 and 10 days after injection. At 10 days post injection, there was a significant trend for bone loss on both sides of the mandible. Periodontal inflammation was associated with alteration in the levels of calcitonin gene-related peptide-like immunoreactivity in nerve terminals innervating the inflamed gingival papilla. Conclusion: Intragingival injection of LPS in the rat provides an easily induced reproducible experimental model of periodontal inflammation that reiterates features of human disease.

引用

页码：596 / 603

页数：8

共 48 条

[1] Effects of capsaicin-induced sensory denervation on osteoclastic resorption in adult rats [J].

Adam, C ;

Llorens, A ;

Baroukh, B ;

Cherruau, M ;

Saffar, JL .

EXPERIMENTAL PHYSIOLOGY, 2000, 85 (01) :61-66

[2] SYNTHESIS OF PROINFLAMMATORY CYTOKINES BY HUMAN GINGIVAL FIBROBLASTS IN RESPONSE TO LIPOPOLYSACCHARIDES AND INTERLEUKIN-1-BETA [J].

AGARWAL, S ;

BARAN, C ;

PIESCO, NP ;

QUINTERO, JC ;

LANGKAMP, HH ;

JOHNS, LP ;

CHANDRA, CS .

JOURNAL OF PERIODONTAL RESEARCH, 1995, 30 (06) :382-389

[3] AGE-RELATED ALTERATIONS OF PERIODONTAL STRUCTURES AROUND CEMENT-ENAMEL JUNCTION AND OF GINGIVAL CONNECTIVE-TISSUE COMPOSITION IN GERMFREE RATS [J].

AMSTADJOSSI, M ;

SCHROEDER, HE .

JOURNAL OF PERIODONTAL RESEARCH, 1978, 13 (01) :76-90

[4] IMMUNOHISTOLOGICAL STUDY OF NEURONAL MARKERS IN INFLAMED GINGIVA OBTAINED FROM CHILDREN WITH DOWNS-SYNDROME [J].

BARRAGHOLME, M ;

MODEER, T ;

LUTHMAN, J .

JOURNAL OF CLINICAL PERIODONTOLOGY, 1991, 18 (08) :624-633

[5] Effect of the sensory neuropeptide antagonists h-CGRP(8-37) and SR 140.33 on pulpal and gingival blood flow in ferrets [J].

Berggreen, E ;

Heyeraas, KJ .

ARCHIVES OF ORAL BIOLOGY, 2000, 45 (07) :537-542

[6] SUBSTANCE-P-IMMUNOREACTIVE AND CGRP-IMMUNOREACTIVE NERVES IN BONE [J].

BJURHOLM, A ;

KREICBERGS, A ;

BRODIN, E ;

SCHULTZBERG, M .

PEPTIDES, 1988, 9 (01) :165-171

[7] CALCITONIN GENE-RELATED PEPTIDE IS A POTENT VASODILATOR [J].

BRAIN, SD ;

WILLIAMS, TJ ;

TIPPINS, JR ;

MORRIS, HR ;

MACINTYRE, I .

NATURE, 1985, 313 (5997) :54-56

[8] Unilateral arthritis: contralateral effects [J].

Donaldson, LF .

TRENDS IN NEUROSCIENCES, 1999, 22 (11) :495-496

[9] Sensory neuropeptide mRNA up-regulation is bilateral in periodontitis in the rat: a possible neurogenic component to symmetrical periodontal disease [J].

El-Aleem, SAA ;

Morales-Aza, BM ;

Donaldson, LF .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2004, 19 (03) :650-658

[10] PERIODONTAL BONE LOSS IN PORPHYROMONAS-GINGIVALIS-INFECTED SPECIFIC PATHOGEN-FREE RATS AFTER PREINOCULATION WITH ENDOGENOUS STREPTOCOCCUS-SANGUIS [J].

FIEHN, NE ;

KLAUSEN, B ;

EVANS, RT .

JOURNAL OF PERIODONTAL RESEARCH, 1992, 27 (06) :609-614

← 1 2 3 4 5 →