Multi layer Films Assembled from Naturally-Derived Materials for Controlled Protein Release

被引:42
作者
Hsu, Bryan B. [1 ,3 ]
Hagerman, Samantha R. [2 ]
Jamieson, Kelsey [2 ]
Veselinovic, Jovana [2 ]
O'Neill, Nicholas [4 ]
Holler, Eggehard [5 ]
Ljubimova, Julia Y. [5 ]
Hammond, Paula T. [2 ,3 ]
机构
[1] MIT, Dept Chem, Cambridge, MA 02139 USA
[2] MIT, Dept Chem Engn, Cambridge, MA 02139 USA
[3] Inst Soldier Nanotechnol, Cambridge, MA 02139 USA
[4] Univ Notre Dame, Dept Chem & Biomol Engn, South Bend, IN 46556 USA
[5] Cedars Sinai Med Ctr, Dept Neurosurg, Nanomed Res Ctr, Los Angeles, CA 90048 USA
关键词
FIBROBLAST GROWTH FACTOR-2; POLYELECTROLYTE MULTILAYERS; MOLECULAR-WEIGHT; EXPONENTIAL-GROWTH; DRUG-RELEASE; THIN-FILMS; DELIVERY; SYSTEMS; LYSOZYME; CHITOSAN;
D O I
10.1021/bm5001839
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Herein we designed and characterized films composed of naturally derived materials for controlled release of proteins. Traditional drug delivery strategies rely on synthetic or semisynthetic materials or utilize potentially denaturing assembly conditions that are not optimal for sensitive biologics. Layer-by-layer (LbL) assembly of films uses benign conditions and can generate films with various release mechanisms including hydrolysis-facilitated degradation. These use components such as synthetic polycations that degrade into non-natural products. Herein we report the use of a naturally derived, biocompatible and degradable polyanion, poly(beta-L-malic acid), alone and in combination with chitosan in an LbL film, whose degradation products of malic acid and chitosan are both generally recognized as safe (GRAS) by the FDA. We have found that films based on this polyanion have shown sustained release of a model protein, lysozyme that can be timed from tens of minutes to multiple days through different film architectures. We also report the incorporation and release of a clinically used biologic, basic fibroblast growth factor (bFGF), which demonstrates the use of this strategy as a platform for controlled release of various biologics.
引用
收藏
页码:2049 / 2057
页数:9
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