Gene and protein expression for prolactin, growth hormone and somatolactin in Sparus aurata: Seasonal variations

被引：25

作者：

Vargas-Chacoff, L. ^{[1
,2
]}

Astola, A. ^{[1
]}

Arjona, F. J. ^{[2
]}

Martin del Rio, M. R. ^{[2
]}

Garcia-Cozar, F. ^{[3
]}

Mancera, J. M. ^{[2
]}

Martinez-Rodriguez, G. ^{[1
]}

机构：

[1] CSIC, Inst Ciencias Marinas Andalucia, E-11510 Puerto Real, Cadiz, Spain

[2] Univ Cadiz, Fac Ciencias Mar & Ambientales, Dept Biol, E-11510 Puerto Real, Cadiz, Spain

[3] Univ Cadiz, Hosp Univ Puerto Real, Dept Bioquim & Biol Mol Immunol, E-11510 Puerto Real, Cadiz, Spain

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2009年 / 153卷 / 01期

关键词：

Growth hormone; Prolactin; Seasonal gene expression; Somatolactin; Sparus aurata; GILTHEAD SEA BREAM; MESSENGER-RNA; NUTRITIONAL-STATUS; SOMATOTROPIC AXIS; FRESH-WATER; RED DRUM; PITUITARY; FISH; GH; TRANSCRIPTION;

D O I：

10.1016/j.cbpb.2009.02.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The seasonal variation of PRL, GH and SL gene and protein expression has been analyzed in gilthead sea bream (Sparus aurata) pituitaries using Real-Time Q-PCR and Western Blots, respectively. Animals were Cultured in earthen ponds under natural photoperiod, temperature and salinity conditions. Samples were taken during winter 2005 (January), spring 2005 (April), Summer 2005 (July) and autumn 2005 (October). beta-actin, used as the housekeeping gene both for Q-RT-PCR and Western analysis, did not present significant differences among seasons. Higher expression was observed during spring and autumn for PRL, summer and winter for GH, and spring for SL. Expression of PRI, GH and SL, presented seasonal variation, suggesting that these hormones could play a role in the molecular signal transduction of environmental factors (especially of photoperiod and temperature) in eurythermal fish. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：130 / 135

页数：6

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