Molecular detection of genogroup I and II picobirnaviruses in pigs in China

被引：17

作者：

Chen, Molin ^{[1
]}

Sun, Huan ^{[1
]}

Lan, Daoliang ^{[2
]}

Hua, Xiuguo ^{[1
]}

Cui, Li ^{[1
]}

Yuan, Congli ^{[1
]}

Yang, Zhibiao ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai Key Lab Vet Biotechnol, Shanghai 200240, Peoples R China

[2] Southwest Univ Nationalities, Tibetan Plateau Base, Chengdu 610041, Peoples R China

来源：

VIRUS GENES | 2014年 / 48卷 / 03期

关键词：

Molecular detection; Picobirnavirus; Pig; China; DOUBLE-STRANDED-RNA; FECAL SPECIMENS; VIRUS; FECES; IDENTIFICATION; DIARRHEA; GENOME;

D O I：

10.1007/s11262-014-1058-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In this study, a total of 187 stool specimens were collected from a pig farm in Hunan province of China, from November 2011 to June 2012. 39 (20.9 %) stool specimens were positive for picobirnaviruses using reverse transcription-polymerase chain reaction. Among 39 stool specimens, 84.6 % (33/39) were identified to be genogroup I (prototype 1-CHN-97), 38.5 % (15/39) belonged to genogroup II (prototype 4-GA-91), and 23.1 % (9/39) of which showed the evidence of genogroup I picobirnavirus were also positive for genogroup II picobirnaviruses. Picobirnaviruses exist in pigs which were divided into five groups according to the age and physiological status. Nineteen representative strains of genogroup I picobirnaviruses and eleven strains of genogroup II picobirnaviruses detected in this study were selected to analyze their phylogenetic relationships with other picobirnaviruses reference strains. The phylogenetic tree analysis suggested the prevalence of multiple picobirnaviruses in pigs in China.

引用

页码：553 / 556

页数：4

共 18 条

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