Synthesis of poly(propylene fumarate)

被引：168

作者：

Kasper, F. Kurtis ^{[1
]}

Tanahashi, Kazuhiro ^{[1
]}

Fisher, John P. ^{[2
]}

Mikos, Antonios G. ^{[1
]}

机构：

[1] Rice Univ, Dept Bioengn, Houston, TX 77251 USA

[2] Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA

来源：

NATURE PROTOCOLS | 2009年 / 4卷 / 04期

基金：

美国国家卫生研究院;

关键词：

CO-ETHYLENE GLYCOL); MARROW STROMAL OSTEOBLASTS; CROSS-LINKING CHARACTERISTICS; IN-VITRO DEGRADATION; TISSUE-ENGINEERED CONSTRUCTS; OSTEOGENIC CELL-POPULATIONS; MECHANICAL-PROPERTIES; VIVO DEGRADATION; POLYMERIC CARRIER; PROPYLENE-GLYCOL;

D O I：

10.1038/nprot.2009.24

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This protocol describes the synthesis of 500-4,000 Da poly(propylene fumarate) (PPF) by a two-step reaction of diethyl fumarate and propylene glycol through a bis(hydroxypropyl) fumarate diester intermediate. Purified PPF can be covalently cross-linked to form degradable polymer networks, which have been widely explored for biomedical applications. The properties of cross-linked PPF networks depend upon the molecular properties of the constituent polymer, such as the molecular weight. The purity of the reactants and the exclusion of water from the reaction system are of utmost importance in the generation of high-molecular-weight PPF products. Additionally, the reaction time and temperature influence the molecular weight of the PPF product. The expected time required to complete this protocol is 3 d.

引用

页码：518 / 525

页数：8

共 60 条

[31] Modification of oligo(poly(ethylene glycol) fumarate) macromer with a GRGD peptide for the preparation of functionalized polymer networks [J].