Synthesis of poly(propylene fumarate)

被引：168
作者：
Kasper, F. Kurtis [1 ]
Tanahashi, Kazuhiro [1 ]
Fisher, John P. [2 ]
Mikos, Antonios G. [1 ]
机构：
[1] Rice Univ, Dept Bioengn, Houston, TX 77251 USA
[2] Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA
来源：
NATURE PROTOCOLS | 2009年 / 4卷 / 04期
基金：
美国国家卫生研究院;
关键词：
CO-ETHYLENE GLYCOL); MARROW STROMAL OSTEOBLASTS; CROSS-LINKING CHARACTERISTICS; IN-VITRO DEGRADATION; TISSUE-ENGINEERED CONSTRUCTS; OSTEOGENIC CELL-POPULATIONS; MECHANICAL-PROPERTIES; VIVO DEGRADATION; POLYMERIC CARRIER; PROPYLENE-GLYCOL;
D O I：
10.1038/nprot.2009.24
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This protocol describes the synthesis of 500-4,000 Da poly(propylene fumarate) (PPF) by a two-step reaction of diethyl fumarate and propylene glycol through a bis(hydroxypropyl) fumarate diester intermediate. Purified PPF can be covalently cross-linked to form degradable polymer networks, which have been widely explored for biomedical applications. The properties of cross-linked PPF networks depend upon the molecular properties of the constituent polymer, such as the molecular weight. The purity of the reactants and the exclusion of water from the reaction system are of utmost importance in the generation of high-molecular-weight PPF products. Additionally, the reaction time and temperature influence the molecular weight of the PPF product. The expected time required to complete this protocol is 3 d.
引用
收藏
页码：518 / 525
页数：8
相关论文
共 60 条
[1]   Three-dimensional culture of differentiating marrow stromal osteoblasts in biomimetic poly(propylene fumarate-co-ethylene glycol)-based macroporous hydrogels [J].
Behravesh, E ;
Mikos, AG .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2003, 66A (03) :698-706
[2]   Adhesion and migration of marrow-derived osteoblasts on injectable in situ crosslinkable poly(propylene fumarate-co-ethylene glycol)-based hydrogels with a covalently linked RGDS peptide [J].
Behravesh, E ;
Zygourakis, K ;
Mikos, AG .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2003, 65A (02) :260-270
[3]   Evaluation of the in vitro degradation of macroporous hydrogels using gravimetry, confined compression testing, and microcomputed tomography [J].
Behravesh, E ;
Timmer, MD ;
Lemoine, JJ ;
Liebschner, MAK ;
Mikos, AG .
BIOMACROMOLECULES, 2002, 3 (06) :1263-1270
[4]   Synthesis of in situ cross-linkable macroporous biodegradable poly(propylene fumarate-co-ethylene glycol) hydrogels [J].
Behravesh, E ;
Jo, S ;
Zygourakis, K ;
Mikos, AG .
BIOMACROMOLECULES, 2002, 3 (02) :374-381
[5]   Biodegradable fumarate-based polyHIPEs as tissue engineering scaffolds [J].
Christenson, Elizabeth M. ;
Soofi, Wafa ;
Holm, Jennifer L. ;
Cameron, Neil R. ;
Mikos, Antonios G. .
BIOMACROMOLECULES, 2007, 8 (12) :3806-3814
[6]   Use of stereolithography to manufacture critical-sized 3D biodegradable scaffolds for bone ingrowth [J].
Cooke, MN ;
Fisher, JP ;
Dean, D ;
Rimnac, C ;
Mikos, AG .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2003, 64B (02) :65-69
[7]  
Coombes Darryl M, 2007, Dent Update, V34, P641
[8]   Poly(propylene fumarate) and poly(DL-lactic-co-glycolic acid) as scaffold materials for solid and foam-coated composite tissue-engineered constructs for cranial reconstruction [J].
Dean, D ;
Topham, NS ;
Meneghetti, SC ;
Wolfe, MS ;
Jepsen, K ;
He, SL ;
Chen, JEK ;
Fisher, JP ;
Cooke, M ;
Rimnac, C ;
Mikos, AG .
TISSUE ENGINEERING, 2003, 9 (03) :495-504
[9]   Effect of transforming growth factor β2 on marrow-infused foam poly(propylene fumarate) tissue-engineered constructs for the repair of critical-size cranial defects in rabbits [J].
Dean, D ;
Wolfe, MS ;
Ahmad, Y ;
Totonchi, A ;
Chen, JEK ;
Fisher, JP ;
Cooke, MN ;
Rimnac, CM ;
Lennon, DP ;
Caplan, AI ;
Topham, NS ;
Mikos, AG .
TISSUE ENGINEERING, 2005, 11 (5-6) :923-939
[10]   THE FORMATION OF PROPYLENE FUMARATE OLIGOMERS FOR USE IN BIOERODIBLE BONE-CEMENT COMPOSITES [J].
DOMB, AJ ;
LAURENCIN, CT ;
ISRAELI, O ;
GERHART, TN ;
LANGER, R .
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY, 1990, 28 (05) :973-985
123456