Genome-Wide Identification, Characterization, and Expression Analyses of P-Type ATPase Superfamily Genes in Soybean

被引:10
作者
Zhao, Bingqian [1 ]
Wu, Haicheng [1 ]
Xu, Wenjing [2 ]
Zhang, Wei [2 ]
Chen, Xi [3 ]
Zhu, Yiyong [4 ]
Chen, Huatao [2 ]
Zeng, Houqing [1 ]
机构
[1] Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 311121, Peoples R China
[2] Jiangsu Acad Agr Sci, Inst Ind Crops, Nanjing 210014, Peoples R China
[3] Nanjing Agr Univ, Coll Life Sci, Dept Biochem & Mol Biol, Nanjing 210095, Peoples R China
[4] Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing 210095, Peoples R China
来源
AGRONOMY-BASEL | 2021年 / 11卷 / 01期
关键词
P-type ATPase; ion pump; phylogenetic analysis; expression analysis; tissue expression pattern; stress; duplication; evolution; cis-acting element; MEMBRANE H+-ATPASE; POLLEN-TUBE GROWTH; PROTON PUMP ATPASE; TRANSCRIPTION FACTORS; AMMONIUM-NUTRITION; BINDING DOMAIN; ION PUMPS; ARABIDOPSIS; FAMILY; TRANSPORT;
D O I
10.3390/agronomy11010071
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
P-type ATPases are transmembrane pumps of cations and phospholipids. They are energized by hydrolysis of ATP and play important roles in a wide range of fundamental cellular and physiological processes during plant growth and development. However, the P-type ATPase superfamily genes have not been characterized in soybean. Here, we performed genome-wide bioinformatic and expression analyses of the P-type ATPase superfamily genes in order to explore the potential functions of P-type ATPases in soybean. A total of 105 putative P-type ATPase genes were identified in the soybean genome. Phylogenetic relationship analysis of the P-type ATPase genes indicated that they can be divided into five subfamilies including P1B, P2A/B, P3A, P4 and P5. Proteins belonging to the same subfamily shared conserved domains. Forty-seven gene pairs were related to segmental duplication, which contributed to the expansion of the P-type ATPase genes during the evolution of soybean. Most of the P-type ATPase genes contained hormonal- and/or stress-related cis-elements in their promoter regions. Expression analysis by retrieving RNA-sequencing datasets suggested that almost all of the P-type ATPase genes could be detected in soybean tissues, and some genes showed tissue-specific expression patterns. Nearly half of the P-type ATPase genes were found to be significantly induced or repressed under stresses like salt, drought, cold, flooding, and/or phosphate starvation. Four genes were significantly affected by rhizobia inoculation in root hairs. The induction of two P2B-ATPase genes, GmACA1 and GmACA2, by phosphate starvation was confirmed by quantitative RT-PCR. This study provides information for understanding the evolution and biological functions of the P-type ATPase superfamily genes in soybean.
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页数:25
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