Overexpression of Mps1 in colon cancer cells attenuates the spindle assembly checkpoint and increases aneuploidy

被引：46

作者：

Ling, Youguo ^{[1
,2
]}

Zhang, Xiaojuan ^{[3
]}

Bai, Yuanyuan ^{[2
]}

Li, Ping ^{[2
]}

Wei, Congwen ^{[2
]}

Song, Ting ^{[2
]}

Zheng, Zirui ^{[2
]}

Guan, Kai ^{[2
]}

Zhang, Yanhong ^{[2
]}

Zhang, Buchang ^{[1
]}

Liu, Xuedong ^{[4
]}

Ma, Runlin Z. ^{[3
]}

Cao, Cheng ^{[2
]}

Zhong, Hui ^{[2
]}

Xu, Quanbin ^{[2
]}

机构：

[1] Anhui Univ, Dept Life Sci, Hefei 230039, Peoples R China

[2] Beijing Inst Biotechnol, Beijing, Peoples R China

[3] Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Dev Biol, Beijing, Peoples R China

[4] Univ Colorado, Boulder, CO 80309 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 450卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Mps1; Overexpression; Spindle assembly checkpoint; Tumorigenesis; Aneuploidy; MITOTIC CHECKPOINT; KINASE; BUBR1; SLIPPAGE; PHOSPHORYLATION; KINETOCHORES; INHIBITION; MITOSIS; ARREST; CDC20;

D O I：

10.1016/j.bbrc.2014.07.071

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The spindle assembly checkpoint kinase Mps1 is highly expressed in several types of cancers, but its cellular involvement in tumorigenesis is less defined. Herein, we confirm that Mps1 is overexpressed in colon cancer tissues. Further, we find that forced expression of Mps1 in the colon cancer cell line SW480 enables cells to become resistant to both Mps1 inhibition-induced checkpoint depletion and cell death. Overexpression of Mps1 also increases genome instability in tumor cells owing to a weakened spindle assembly checkpoint. Collectively, our findings suggest that high levels of Mps1 contribute to tumorigenesis by attenuating the spindle assembly checkpoint. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：1690 / 1695

页数：6

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