Fluorogen-Peptide Conjugates with Tunable Aggregation-Induced Emission Characteristics for Bioprobe Design

被引：45

作者：

Zhang, Ruoyu ^{[1
]}

Yuan, Youyong ^{[1
]}

Liang, Jing ^{[1
]}

Kwok, Ryan T. K. ^{[2
]}

Zhu, Qing ^{[3
]}

Feng, Guangxue ^{[1
]}

Geng, Junlong ^{[1
]}

Tang, Ben Zhong ^{[2
,4
]}

Liu, Bin ^{[1
,5
]}

机构：

[1] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117576, Singapore

[2] Hong Kong Univ Sci & Technol, Div Biomed Engn, Dept Chem, Kowloon, Hong Kong, Peoples R China

[3] Zhejiang Univ Technol, Inst Bioengn, Hangzhou 310014, Zhejiang, Peoples R China

[4] S China Univ Technol, State Key Lab Luminescent Mat & Devices, Guangdong Innovat Res Team, SCUT HKUST Joint Res Lab, Guangzhou 510640, Guangdong, Peoples R China

[5] Inst Mat Res Engn, Singapore 117602, Singapore

来源：

ACS APPLIED MATERIALS & INTERFACES | 2014年 / 6卷 / 16期

基金：

新加坡国家研究基金会;

关键词：

light-up probe; aggregation-induced emission; fluorescent probes; thiol detection; tetraphenylethene; TURN-ON DETECTION; WATER PARTITION-COEFFICIENT; LIGHT-UP BIOPROBE; FLUORESCENT-PROBES; CHEMICAL SENSORS; CELL APOPTOSIS; ACTIVITY ASSAY; CANCER-CELLS; GLUTATHIONE; CYSTEINE;

D O I：

10.1021/am5036048

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Fluorogens with aggregation-induced emission (AIE) characteristics are attracting intense research interest, and an AIE-peptide conjugate strategy has been reported for developing turn-on probes based on hydrophilic peptide ligands. To build a model also suitable for hydrophobic ligands, we propose to fine-tune the AIE characteristics for probe design. In this work, an iconic AIE fluorogen tetraphenylethene (TPE) was designed to conjugate with peptide fragments containing different numbers of aspartic acid (D) units. Relationships between the numbers of D and the hydrophilicity, optical properties, and aggregate sizes and the ATE characteristics of TPE-peptide conjugates were investigated carefully. Five carboxyl groups were found to be the threshold to "turn off" the fluorescence of TPE. As a proof-of-concept, TPE-SS-D-5 containing a cleavable disulfide bond was synthesized for thiol turn-on detection. The validated tunable AIE characteristic offers new opportunities to design fluorescence turn-on probes based on hydrophobic recognition elements and AIE fluorogens.

引用

页码：14302 / 14310

页数：9

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