Glutamine deamidation: an indicator of antiquity, or preservational quality?

被引：77

作者：

Schroeter, Elena R. ^{[1
]}

Cleland, Timothy P. ^{[2
]}

机构：

[1] N Carolina State Univ, Dept Biol Sci, Raleigh, NC 27695 USA

[2] Rensselaer Polytech Inst, Dept Biomed Engn, Troy, NY 12182 USA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2016年 / 30卷 / 02期

关键词：

ASPARAGINYL RESIDUES; PROTEIN DEAMIDATION; RATES; PEPTIDES; COLLAGEN; CHROMATOGRAPHY; EVOLUTION; SEQUENCE; HISTORY; HAZARD;

D O I：

10.1002/rcm.7445

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

RATIONALE: Much credence has been given in the paleoproteomic community to glutamine deamidation as a proxy for the age of proteins derived from fossil and subfossil material, and this modification has been invoked as a means for determining the endogeneity of molecules recovered from very old fossil specimens. METHODS: We re-evaluated the relationship between glutamine deamidation and geologic time by examining previously published data from five recent mass spectrometry studies of archeaological fossils. Deamidation values recovered for fossils were graphed against their reported chronologic age using WebPlotDigitizer. RESULTS: The experimental data that has been produced from fossil material to date show that the extent of glutamine deamidation does not correspond to the absolute age of the specimens being examined, but rather show extreme variation between specimens of similar age and taxonomic affinity. CONCLUSIONS: Because deamidation rates and levels can be greatly affected by numerous chemical and environmental factors, we propose that glutamine deamidation is better suited as an indicator of preservational quality and/or environmental conditions than a mark of the endogeneity or authenticity of ancient proteins. Copyright (c) 2015 John Wiley & Sons, Ltd.

引用

页码：251 / 255

页数：5

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