A protein biosensor for lactate

被引：24
作者：
D'Auria, S
Gryczynski, Z
Gryczynski, I
Rossi, M
Lakowicz, JR
机构：
[1] Univ Maryland, Ctr Fluorescence Spect, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA
[2] CNR, Inst Prot Biochem & Enzymol, I-80125 Naples, Italy
来源：
ANALYTICAL BIOCHEMISTRY | 2000年 / 283卷 / 01期
关键词：
D O I：
10.1006/abio.2000.4611
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Blood lactate is a clinically valuable diagnostic indicator. In this preliminary report we describe a protein biosensor for L-lactate based on beef heart lactate dehydrogenase (LDH). LDH was noncovalently labeled with 8-anilino-1-naphthalene sulfonic acid (ANS). The ANS-labeled LDH displayed an approximately 40% decrease in emission intensity upon binding lactate. This decrease can be used to measure the lactate concentration. The ANS-labeled LDH was further utilized in a new sensing format, polarization sensing, which is suitable for miniaturization to a point-of-care lactate monitor. However, temporal instability of beef heart LDH indicates the need for further protein engineering prior to development of a more robust lactatesensing protein. (C) 2000 Academic Press.
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收藏
页码：83 / 88
页数：6
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