Dynamic probing of nucleic acid-protein interactions by biphotonic laser chemistry

被引：0
|
作者：
Vidolova-Angelova, E. P. [1 ]
Peshev, Z. [2 ]
Angelov, D. A. [3 ]
机构：
[1] Bulgarian Acad Sci, Inst Solid State Phys, BU-1784 Sofia, Bulgaria
[2] Bulgarian Acad Sci, Inst Elect, BU-1784 Sofia, Bulgaria
[3] Univ Lyon, Ecole Normale Super Lyon, LMBC, CNRS UMR 5239, F-69364 Lyon 07, France
来源：
JOURNAL OF OPTOELECTRONICS AND ADVANCED MATERIALS | 2009年 / 11卷 / 09期
关键词：
DNA-transcription factors interactions; NF-kappa B; Biphotonic laser chemistry; UV laser footprinting; NF-KAPPA-B; DNA; PHOTOLYSIS; COMPLEX;
D O I：
暂无
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
In this study, we describe a new approach for a millisecond dynamic study with high spatial resolution of non-equilibrium protein-DNA interactions in solution The approach is based on mapping the time-resolved UV laser-induced biphotonic DNA oxidative lesions during DNA recognition and binding of a specific protein Our laser "photofootprinting" approach was applied for studying the interaction of the human necrosis factor NF-kappa B p50 homodimer bound to a 37 base pair DNA Evidence is provided for the occurrence of a two-step process rapid (30 ms) formation of the pre-equilibrium complex and slow (1 s) protein rearrangement and DNA conformation accommodation.
引用
收藏
页码：1198 / 1201
页数：4
相关论文
共 50 条
  • [1] NUCLEIC ACID-PROTEIN INTERACTIONS IN A SIMPLE ISOMETRIC VIRUS
    KAPER, JM
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1969, (SEP): : B272 - &
  • [2] Impacts of Molecular Structure on Nucleic Acid-Protein Interactions
    Bowater, Richard P.
    Brazda, Vaclav
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (01)
  • [3] THE TIGHTNESS OF NUCLEIC ACID-PROTEIN INTERACTIONS AND SYNTHESIS OF NUCLEIC-ACIDS AND PROTEIN IN CELL
    SVIRNOVSKY, AI
    TSYGANKOV, VG
    AGEICHIK, VM
    SHIMANSKAYA, TV
    VOPROSY MEDITSINSKOI KHIMII, 1991, 37 (01): : 16 - 19
  • [4] Dissecting nucleic acid-protein interactions using challenge phage
    Maloy, Stanley R.
    Gardner, Jeffrey
    ADVANCED BACTERIAL GENETICS: USE OF TRANSPOSONS AND PHAGE FOR GENOMIC ENGIEERING, 2007, 421 : 227 - 249
  • [5] Energy Transfer as A Driving Force in Nucleic Acid-Protein Interactions
    Zavyalova, Elena
    Kopylov, Alexey
    MOLECULES, 2019, 24 (07)
  • [6] Affinity characterization of nucleic acid-protein interactions by SpotMatrix SPR technology
    Yan, YX
    Baggio, R
    Lambert, J
    Olesen, C
    Xue, Y
    Ferrell, K
    Wassaf, D
    Carlson, N
    Palmer, M
    Arenas, J
    FASEB JOURNAL, 2004, 18 (08): : C203 - C203
  • [7] Development of a Quantitative BRET Affinity Assay for Nucleic Acid-Protein Interactions
    Vickers, Timothy A.
    Crooke, Stanley T.
    PLOS ONE, 2016, 11 (08):
  • [8] NMR and nucleic acid-protein interactions: The Lac repressor operator system
    Kaptein, R
    Boelens, R
    Chuprina, VP
    Rullmann, JAC
    Slijper, M
    NUCLEAR MAGNETIC RESONANCE AND NUCLEIC ACIDS, 1995, 261 : 513 - 524
  • [9] Contributions of mass spectrometry in the study of nucleic acid-binding proteins and of nucleic acid-protein interactions
    Rusconi, F
    Guillonneau, F
    Praseuth, D
    MASS SPECTROMETRY REVIEWS, 2002, 21 (05) : 305 - 348
  • [10] The nucleic acid-protein compounds.
    Steudel, H
    Peiser, E
    HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1922, 122 (4/6): : 298 - 306
12345