Neurogranin expression in stably transfected N2A cell line affects cytosolic calcium level by nitric oxide stimulation

被引：8
作者：
Yang, HM
Lee, PHH
Lim, TM
Sheu, FS
机构：
[1] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore
[2] Natl Univ Singapore, Univ Scholars Program, Singapore 119260, Singapore
来源：
MOLECULAR BRAIN RESEARCH | 2004年 / 129卷 / 1-2期
关键词：
neurogranin; phosphorylation; nitric oxide; oxidation; cytosolic-free calcium concentration; calmodulin-binding protein;
D O I：
10.1016/j.molbrainres.2004.06.039
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
To test a cellular effect of rodent neurogranin (Ng) oxidation as compared to Ng phosphorylation, we develop a cell model capable of stable expression of Ng using the Tet-On system, and determine whether Ng oxidation regulates intracellular calcium level. Our results show that Ng oxidation by nitric oxide donor induces an increase of [Ca2+](i) in Ng-expressed cells as compared to the control cells without expressing Ng. These results suggest that Ng oxidation plays a significant role in intracellular Ca2+ homeostasis, essential for the activated signaling networks in learning and memory. (C) 2004 Elsevier B.V. All rights reserved.
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收藏
页码：171 / 178
页数：8
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