In vitro antiplasmodial activity of plants used in Benin in traditional medicine to treat malaria

被引:91
作者
Bero, Joanne [1 ]
Ganfon, Habib [1 ,5 ]
Jonville, Marie-Caroline [2 ]
Frederich, Michel [2 ]
Gbaguidi, Fernand [3 ]
DeMol, Patrick [4 ]
Moudachirou, Mansourou [3 ,5 ]
Quetin-Leclercq, Joelle [1 ]
机构
[1] Univ Catholique Louvain, Louvain Drug Res Inst, UCL 7230, B-1200 Brussels, Belgium
[2] Univ Liege, Drug Res Ctr, Lab Pharmacognoise, B-4000 Liege, Belgium
[3] Ctr Beninois Rech Sci & Tech, Cotonou, Benin
[4] Univ Liege, CIRM, Lab Microbiol Med, B-4000 Liege, Belgium
[5] Univ Abomey Calavi, Fac Sci Sante, Lab Pharmacognosie & Huiles Essentelles UFR Pharm, Cotonou, Benin
关键词
Antiplasmodial activity; Traditional medicine; Benin; Acanthospermum hispidum; Keetia leucantha; Carpolobia lutea; Strychnos spinosa; ANTITRYPANOSOMAL ACTIVITY; ACANTHOSPERMUM-HISPIDUM; PLASMODIUM-FALCIPARUM; HELIOTROPIUM-INDICUM; TRICHILIA-EMETICA; STRYCHNOS-SPINOSA; AFRICAN PLANTS; BURKINA-FASO; ALKALOIDS; EXTRACTS;
D O I
10.1016/j.jep.2009.02.004
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Aim of the study: The aim of the study was to evaluate the in vitro antiplasmodial activity of crude extracts of 12 plant species traditionally used in Benin for the treatment of malaria in order to validate their use. Materials and methods: For each species, dichloromethane, methanol and total aqueous extracts were tested. The antiplasmodial activity of extracts was evaluated using the measurement of the plasmodial lactate dehydrogenase activity on chloroquine-sensitive (3D7) and resistant (W2) strains of Plasmodium falciparum. The selectivity of the different extracts was evaluated using the MTT test on J774 macrophage-like murine cells and W138 human normal fibroblasts. Results: The best growth inhibition of both strains of Plasmodium falciparum was observed with the dichloromethane extracts of Acanthospermum hispidum DC. (Asteraceae) (IC(50) = 7.5 mu g/ml on 3D7 and 4.8 mu g/ml on W2), Keetia leucantha (K. Krause) Bridson (syn. Plectronia leucantha Krause) (Rubiaceae) leaves and twigs (IC(50) = 13.8 and 11.3 mu g/ml on 3D7 and IC(50) = 26.5 and 15.8 mu g/ml on W2, respectively), Carpolobia lutea G.Don. (Polygalaceae) (IC(50) = 19.4 mu g/ml on 3D7 and 8.1 mu g/ml on W2) and Strychnos spinosa Lam. (Loganiaceae) leaves (IC(50) = 15.6 mu g/ml on 3D7 and 8.9 mu g/ml on W2). All these extracts had a low cytotoxicity. Conclusion: Our study gives some justifications for the traditional uses of some investigated plants. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:439 / 444
页数:6
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