Detection of human-derived fecal contamination in Puerto Rico using carbamazepine, HF183 Bacteroides, and fecal indicator bacteria

被引：12

作者：

Wade, Christina ^{[1
]}

Otero, Ernesto ^{[1
]}

Poon-Kwong, Brennan ^{[2
]}

Rozier, Ralph ^{[2
]}

Bachoon, Dave ^{[2
]}

机构：

[1] Univ Puerto Rico, Dept Marine Sci, Mayaguez, PR 00681 USA

[2] Georgia Coll & State Univ, Dept Biol & Environm Sci, Milledgeville, GA 31061 USA

来源：

MARINE POLLUTION BULLETIN | 2015年 / 101卷 / 02期

关键词：

Carbamazepine; Chemical fecal indicators; Caribbean; E; coli; Pharmaceutical; Puerto Rico; WASTE-WATER TREATMENT; SEWAGE-TREATMENT PLANTS; PERSONAL CARE PRODUCTS; REAL-TIME PCR; ESCHERICHIA-COLI; DRINKING-WATER; AQUATIC ENVIRONMENT; COASTAL WATERS; SURFACE WATERS; PHARMACEUTICALS;

D O I：

10.1016/j.marpolbul.2015.11.016

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The level of fecal pollution in 17 sites in Puerto Rico was determined by Escherichia coli (E. coli) enumeration using an enzyme substrate medium and Quanti-Tray (R)/2000. Human fecal pollution was identified using an enzyme-linked immunosorbent assay for the detection of carbamazepine (CBZ) and quantitative polymerase chain reaction (qPCR) detection of the human Bacteroides marker, HF183. Carbamazepine was detected in 16 out of 17 sites, including Condado Lagoon, a popular recreational area. Elevated E. coli levels (>410 CFU 100 mL(-1)) were detected in 13 sites. Average CBZ concentrations ranged from 0.005 mu g L-1 to 0.482 mu g L-1 and 7 sites were positive for HF183. Higher CBZ concentrations were associated with the detection of HF183 (Mann-Whitney test; U = 42.0; df = 7; 1-tailed P value = 0.013). This was the second study to determine surface water concentrations of CBZ in the Caribbean and the first in Puerto Rico. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：872 / 877

页数：6

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