A putative branched-chain-amino-acid transaminase gene required for HC-toxin biosynthesis and pathogenicity in Cochliobolus carbonum

被引：29

作者：

Cheng, YQ ^{[1
]}

Ahn, JH ^{[1
]}

Walton, JD ^{[1
]}

机构：

[1] Michigan State Univ, Dept Energy, Plant Res Lab, E Lansing, MI 48824 USA

来源：

MICROBIOLOGY-UK | 1999年 / 145卷

关键词：

Cochliobolus (Helminthosporium) carbonum; cyclic peptide; branched-chain-amino-acid transaminase; TOXF; HC-toxin;

D O I：

10.1099/00221287-145-12-3539

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The cyclic tetrapeptide HC-toxin is required for pathogenicity of the filamentous fungus Cochliobolus carbonum on maize. HC-toxin production is controlled by a complex locus, TOX2. The isolation and characterization of a new gene of the TOX2 locus, TOXF. is reported. It is shown that TOXF is specifically required for HC-toxin production and pathogenicity. It is present as two or three copies in all HC-toxin-producing (Tox2(+)) isolates and is absent in toxin-non-producing strains. The deduced amino acid sequence of TOXF has moderate homology to many known or putative branched-chain-amino-acid transaminases from various species. A strain of C. carbonum with all copies of TOXF disrupted grew normally but lost HC-toxin production and pathogenicity. It is proposed that TOXF has a biosynthetic role in HC-toxin synthesis, perhaps to aminate a precursor of Aeo (2-amino-9,10-epoxi-8-oxodecanoic acid).

引用

页码：3539 / 3546

页数：8

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