Diurnal Oscillations in Liver Mass and Cell Size Accompany Ribosome Assembly Cycles

被引：154

作者：

Sinturel, Flore ^{[1
,8
]}

Gerber, Alan ^{[1
,9
]}

Mauvoisin, Daniel ^{[2
,3
]}

Wang, Jingkui ^{[2
,4
]}

Gatfield, David ^{[5
]}

Stubblefield, Jeremy J. ^{[6
]}

Green, Carla B. ^{[6
]}

Gachon, Frederic ^{[3
,7
]}

Schibler, Ueli ^{[1
]}

机构：

[1] Univ Geneva, Sci 3, Dept Mol Biol, iGE3, CH-1211 Geneva, Switzerland

[2] Ecole Polytech Fed Lausanne, Sch Life Sci, Inst Bioengn, CH-1015 Lausanne, Switzerland

[3] Nestle Inst Hlth Sci, Dept Diabet & Circadian Rhythms, CH-1015 Lausanne, Switzerland

[4] IMP Res Inst Mol Pathol, Campus Vienna Bioctr 1, A-1030 Vienna, Austria

[5] Univ Lausanne, Ctr Integrat Genom, CH-1015 Lausanne, Switzerland

[6] Univ Texas Southwestern Med Ctr Dallas, Dept Neurosci, Dallas, TX 75390 USA

[7] Ecole Polytech Fed Lausanne, Sch Life Sci, CH-1015 Lausanne, Switzerland

[8] Univ Geneva, Fac Med, Diabet Ctr, Dept Cellular Physiol & Metab,iGE3, Geneva, Switzerland

[9] Rockefeller Univ, Lab Biochem & Mol Biol, New York, NY 10065 USA

来源：

CELL | 2017年 / 169卷 / 04期

基金：

瑞士国家科学基金会; 欧洲研究理事会;

关键词：

CIRCADIAN TRANSCRIPTION; POLY(A) POLYMERASE; RNA DEGRADATION; MESSENGER-RNA; POLYADENYLATION; RHYTHMS; CLOCK; SURVEILLANCE; ADENYLATION; METABOLISM;

D O I：

10.1016/j.cell.2017.04.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The liver plays a pivotal role in metabolism and xenobiotic detoxification, processes that must be particularly efficient when animals are active and feed. A major question is how the liver adapts to these diurnal changes in physiology. Here, we show that, in mice, liver mass, hepatocyte size, and protein levels follow a daily rhythm, whose amplitude depends on both feeding-fasting and light-dark cycles. Correlative evidence suggests that the daily oscillation in global protein accumulation depends on a similar fluctuation in ribosome number. Whereas rRNA genes are transcribed at similar rates throughout the day, some newly synthesized rRNAs are polyadenylated and degraded in the nucleus in a robustly diurnal fashion with a phase opposite to that of ribosomal protein synthesis. Based on studies with cultured fibroblasts, we propose that rRNAs not packaged into complete ribosomal subunits are polyadenylated by the poly(A) polymerase PAPD5 and degraded by the nuclear exosome.

引用

页码：651 / 663

页数：13

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