Highly sensitive detection using laser two-photon excited fluorescence in capillary electrophoresis

被引：9

作者：

Song, JM

Inoue, T

Kawazumi, H

Ogawa, T

机构：

[1] KYUSHU UNIV,DEPT MOL SCI & TECHNOL,KASUGA,FUKUOKA 816,JAPAN

[2] KINKI UNIV,DEPT IND CHEM,IIZUKA,FUKUOKA 820,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 765卷 / 02期

关键词：

detection; capillary electrophoresis; two-photon laser-induced fluorescence; fluorescence detection; two-photon; 7-amino-4-methylcoumarine (coumarine 440); 7-diethylaminocoumarine 3-carboxylic acid succinimidyl ester (DCCS);

D O I：

10.1016/S0021-9673(96)00937-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The laser two-photon excited fluorescence (LTPF) has been applied to a capillary electrophoresis (CE) system using a self mode-locked titanium-sapphire laser. The analytes were coumarine 440 (7-amino-4-methyl coumarine) and DCCS (7-diethyl aminocoumarine-3-carboxylic acid succimmidyl ester). The two-photon excited fluorescence emission spectrum of DCCS was identical to the one-photon excited fluorescence emission spectrum. The LTPF quantum efficiency increased through the addition of methanol to the buffer solution, and, as a result, the detection limit decreased. The detection limit of coumarine 440 was 8.0 amol, this value was as low as that obtained by one-photon excited fluorescence.

引用

页码：315 / 319

页数：5

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