Purification and characterization of a novel cis-epoxysuccinate hydrolase from Klebsiella sp. that produces L(+)-tartaric acid

被引：12

作者：

Cheng, Yongqing ^{[1
]}

Wang, Li ^{[1
]}

Pan, Haifeng ^{[1
,2
]}

Bao, Wenna ^{[2
]}

Sun, Weirong ^{[2
]}

Xie, Zhipeng ^{[1
,2
]}

Zhang, Jianguo ^{[1
,2
]}

Zhao, Yuhua ^{[3
]}

机构：

[1] Zhejiang Univ, Inst Biochem, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China

[2] Hangzhou Bioking Biochem Engn Co Ltd, Hangzhou 311106, Zhejiang, Peoples R China

[3] Zhejiang Univ, Inst Microbiol, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2014年 / 36卷 / 11期

基金：

中国国家自然科学基金;

关键词：

cis-Epoxysuccinate hydrolase; Epoxide hydrolase; L(+)-Tartaric acid; Klebsiella sp; EPOXIDE HYDROLASE; ENZYME;

D O I：

10.1007/s10529-014-1614-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A strain of Klebsiella sp. BK-58 that produces cis-epoxysuccinate was screened and identified. This novel enzyme hydrolyzes cis-epoxysuccinate to l(+)-tartaric acid and was purified to homogeneity. Its molecular mass was 30.1 kDa determined by MALDI-TOF-MS analysis. It was stable up to 50 A degrees C and from pH 5 to 11 with optima at 50 A degrees C and pH 8.5. The enzyme was metal-independent and was strongly inhibited by 1 mM Cu2+ and Ag+, and 1 % (w/v) SDS. The K (m) , V (max) and turnover number (k (cat) ) values for cis-epoxysuccinate were 19.3, 2.24 mM min(-1) and 220 s(-1), respectively.

引用

页码：2325 / 2330

页数：6

共 9 条

[1] Immobilization of a whole-cell epoxide-hydrolyzing biocatalyst in sodium alginate-cellulose sulfate-poly(methylene-co-guanidine) capsules using a controlled encapsulation process [J].

Bucko, M ;

Vikartovská, A ;

Lacík, I ;

Kolláriková, G ;

Gemeiner, P ;

Pätoprsty, V ;

Brygin, M .

ENZYME AND MICROBIAL TECHNOLOGY, 2005, 36 (01) :118-126

[2]

de Vires EJ, 2003, CURR OPIN BIOTECH, V14, P414

[3]

Kamatani Y., 1977, U.S. Patent, Patent No. [US4011135, 4011135]

[4] Cloning, sequencing, and expression of a novel epoxide hydrolase gene from Rhodococcus opacus in Escherichia coli and characterization of enzyme [J].

Liu, Zhiqiang ;

Li, Yin ;

Xu, Yingying ;

Ping, Lifeng ;

Zheng, Yuguo .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 74 (01) :99-106

[5] Isolation and identification of a novel Rhodococcus sp ML-0004 producing epoxide hydrolase and optimization of enzyme production [J].

Liu, Zhiqiang ;

Li, Yin ;

Ping, Lifeng ;

Xu, Yingying ;

Cui, Fengjie ;

Xue, Yaping ;

Zheng, Yuguo .

PROCESS BIOCHEMISTRY, 2007, 42 (05) :889-894

[6] Microbial epoxide hydrolases for preparative biotransformations [J].

Steinreiber, A ;

Faber, K .

CURRENT OPINION IN BIOTECHNOLOGY, 2001, 12 (06) :552-558

[7]

Tsurumi Y., 1978, U.S. Patent, Patent No. [US4092220, 4092220]

[8]

Wang ZQ, 2012, APPL MICROBIOL BIOT, V97, P2433

[9] Continuous production of L(+)-tartaric acid from cis-epoxysuccinate using a membrane recycle reactor [J].

Willaert, Ronnie ;

De Vuyst, Luc .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 71 (02) :155-163

← 1 →