Controlled assembly: A prerequisite for the use of recombinant spider silk in regenerative medicine?

被引：32

作者：

Rising, Anna ^{[1
,2
]}

机构：

[1] Karolinska Inst, KI Alzheimer Dis Res Ctr, NVS Neurobiol Care Sci & Soc Dept, S-14186 Huddinge, Sweden

[2] Swedish Univ Agr Sci, Dept Anat Physiol & Biochem, S-75123 Uppsala, Sweden

来源：

ACTA BIOMATERIALIA | 2014年 / 10卷 / 04期

关键词：

Spidroin; Protein; Assembly; Recombinant; Scaffold; MAJOR AMPULLATE SILK; DRAGLINE SILK; MECHANICAL-PROPERTIES; EUPROSTHENOPS-AUSTRALIS; WASP SPIDER; PROTEIN; NMR; FIBERS; MICROSTRUCTURE; ORGANIZATION;

D O I：

10.1016/j.actbio.2013.09.030

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Recent biotechnological progress has enabled the production of spider silk proteins, spidroins, in heterologous hosts. Matrices based on recombinant spidroins support stem cell growth and are well tolerated when implanted in living tissue, thus the material is highly attractive for use in regenerative medicine. However, the matrices made are far from natural silk in terms of mechanical properties and are either spontaneously assembled, which results in heterogeneous products, or spun from harsh solvents with the concomitant risk of harmful remnants in the final products. If we could mimic the spider's aqueous silk spinning process we would likely obtain a material that had reproducible and better characteristics and that more easily could be transferred to clinical practice. Herein, the knowledge of the spiders' silk production system and the prerequisites for artificial spinning and assembly of recombinant proteins are reviewed and discussed in a biomedical context. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：1627 / 1631

页数：5

共 75 条

[1] Three-dimensional scaffold made from recombinant spider silk protein for tissue engineering [J].

Agapov, I. I. ;

Pustovalova, O. L. ;

Moisenovich, M. M. ;

Bogush, V. G. ;

Sokolova, O. S. ;

Sevastyanov, V. I. ;

Debabov, V. G. ;

Kirpichnikov, M. P. .

DOKLADY BIOCHEMISTRY AND BIOPHYSICS, 2009, 426 (01) :127-130

[2] Spider silk fibres in artificial nerve constructs promote peripheral nerve regeneration [J].

Allmeling, C. ;

Jokuszies, A. ;

Reimers, K. ;

Kall, S. ;

Choi, C. Y. ;

Brandes, G. ;

Kasper, C. ;

Scheper, T. ;

Guggenheim, M. ;

Vogt, P. M. .

CELL PROLIFERATION, 2008, 41 (03) :408-420

[3] The influence of specific binding of collagen-silk chimeras to silk biomaterials on hMSC behavior [J].

An, Bo ;

DesRochers, Teresa M. ;

Qin, Guokui ;

Xia, Xiaoxia ;

Thiagarajan, Geetha ;

Brodsky, Barbara ;

Kaplan, David L. .

BIOMATERIALS, 2013, 34 (02) :402-412

[4] Morphology and Composition of the Spider Major Ampullate Gland and Dragline Silk [J].

Andersson, Marlene ;

Holm, Lena ;

Ridderstrale, Yvonne ;

Johansson, Jan ;

Rising, Anna .

BIOMACROMOLECULES, 2013, 14 (08) :2945-2952

[5] Self-assembly of spider silk proteins is controlled by a pH-sensitive relay [J].

Askarieh, Glareh ;

Hedhammar, My ;

Nordling, Kerstin ;

Saenz, Alejandra ;

Casals, Cristina ;

Rising, Anna ;

Johansson, Jan ;

Knight, Stefan D. .

NATURE, 2010, 465 (7295) :236-U125

[6]

Augsten K, 2000, SCANNING, V22, P12

[7] Multiple recombining loci encode MaSp1, the primary constituent of dragline silk, in widow spiders (Latrodectus: Theridiidae) [J].

Ayoub, Nadia A. ;

Hayashi, Cheryl Y. .

MOLECULAR BIOLOGY AND EVOLUTION, 2008, 25 (02) :277-286

[8] Blueprint for a High-Performance Biomaterial: Full-Length Spider Dragline Silk Genes [J].

Ayoub, Nadia A. ;

Garb, Jessica E. ;

Tinghitella, Robin M. ;

Collin, Matthew A. ;

Hayashi, Cheryl Y. .

PLOS ONE, 2007, 2 (06)

[9] RGD-functionalized bioengineered spider dragline silk biomaterial [J].

Bini, Elisabetta ;

Foo, Cheryl Wong Po ;

Huang, Jia ;

Karageorgiou, Vassilis ;

Kitchel, Brandon ;

Kaplan, David L. .

BIOMACROMOLECULES, 2006, 7 (11) :3139-3145

[10] A Novel Model System for Design of Biomaterials Based on Recombinant Analogs of Spider Silk Proteins [J].

Bogush, Vladimir G. ;

Sokolova, Olga S. ;

Davydova, Lyubov I. ;

Klinov, Dmitri V. ;

Sidoruk, Konstantin V. ;

Esipova, Natalya G. ;

Neretina, Tatyana V. ;

Orchanskyi, Igor A. ;

Makeev, Vsevolod Yu ;

Tumanyan, Vladimir G. ;

Shaitan, Konstantin V. ;

Debabov, Vladimir G. ;

Kirpichnikov, Mikhail P. .

JOURNAL OF NEUROIMMUNE PHARMACOLOGY, 2009, 4 (01) :17-27

← 1 2 3 4 5 6 7 8 →