MISER chiral supercritical fluid chromatography for high throughput analysis of enantiopurity

被引：40

作者：

Zawatzky, Kerstin ^{[1
]}

Biba, Mirlinda ^{[1
]}

Regalado, Erik L. ^{[1
]}

Welch, Christopher J. ^{[1
]}

机构：

[1] Merck Res Labs, Dept Proc & Analyt Chem, Rahway, NJ USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2016年 / 1429卷

关键词：

Multiple Injections in a Single Experimental; Run; Supercritical fluid chromatography; High throughput analysis; Chiral separation; Enantiopurity analysis; Ultrafast enantioseparation; LIQUID-CHROMATOGRAPHY; PHARMACEUTICAL COMPOUNDS; MASS-SPECTROMETRY; STATIONARY PHASES; SEPARATIONS; HPLC; PERFORMANCE; PARTICLES; DISCOVERY; REMOVAL;

D O I：

10.1016/j.chroma.2015.12.057

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MISER chromatographic analysis (Multiple Injections in a Single Experimental Run) using supercritical fluid chromatography (SFC) with pressurized carbon dioxide-based eluents is well suited to the high throughput analysis of enantiopurity. SFC is currently the preferred method for fast enantiopurity analysis, with analysis times of only a few seconds achievable in some cases. Injector programming using both the Agilent Infinity and Shimadzu Nexera UC instruments permitted MISER SFC experiments to be performed. Several case studies are presented, showcasing the power and versatility of the technique, with 'plate analysis times' (the time required for analysis of enantiopurity of 96 samples) of less than 33-34min achievable in the best cases. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：374 / 379

页数：6

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