pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis

被引：38

作者：

Orikasa, Yoshitake ^{[1
]}

Tanaka, Mika ^{[1
]}

Sugihara, Shinji ^{[1
]}

Hori, Ryuji ^{[1
,2
]}

Nishida, Takanori ^{[1
]}

Ueno, Akio ^{[1
]}

Morita, Naoki ^{[3
]}

Yano, Yutaka ^{[4
]}

Yamamoto, Kouhei ^{[5
]}

Shibahara, Akira ^{[5
]}

Hayashi, Hidenori ^{[6
,7
]}

Yamada, Yohko ^{[8
]}

Yamada, Akiko ^{[9
]}

Yu, Reiko ^{[9
]}

Watanabe, Kazuo ^{[9
]}

Okuyama, Hidetoshi ^{[1
,2
]}

机构：

[1] Hokkaido Univ, Lab Environm Mol Biol, Fac Environm Earth Sci, Sapporo, Hokkaido 0600810, Japan

[2] Hokkaido Univ, Fac Sci, Sapporo, Hokkaido 0600810, Japan

[3] Natl Inst Adv Ind Sci & Technol, Res Inst Genome Based Biofactory, Sapporo, Hokkaido, Japan

[4] Fisheries Res Agcy, Natl Res Inst Fisheries Sci, Yokohama, Kanagawa, Japan

[5] Osaka Prefecture Univ, Dept Clin Nutr, Habikino, Japan

[6] Ehime Univ, Cell Free Sci & Technol Res Ctr, Matsuyama, Ehime, Japan

[7] Ehime Univ, Satellite Venture Business Lab, Matsuyama, Ehime, Japan

[8] Japan Womens Univ, Dept Chem & Biol Sci, Fac Sci, Tokyo 112, Japan

[9] Sagami Chem Res Ctr, Ayase, Kanagawa, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2009年 / 295卷 / 02期

关键词：

docosahexaenoic acid; eicosapentaenoic acid; Moritella marina strain MP-1; polyunsaturated fatty acid; pfaB gene; PHOSPHOPANTETHEINYL TRANSFERASE GENE; PHOTOBACTERIUM-PROFUNDUM SS9; MARINA STRAIN MP-1; EICOSAPENTAENOIC-ACID; DOCOSAHEXAENOIC ACID; ESCHERICHIA-COLI; DEEP-SEA; SP-NOV; PROKARYOTES; INTESTINES;

D O I：

10.1111/j.1574-6968.2009.01582.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

When pDHA4, a vector carrying all five pfaA-pfaE genes responsible for docosahexaenoic acid (DHA; 22:6) biosynthesis in Moritella marina MP-1, was coexpressed in Escherichia coli with the individual pfaA-pfaD genes for eicosapentaenoic acid (EPA; 20:5) biosynthesis from Shewanella pneumatophori SCRC-2738, both polyunsaturated fatty acids were synthesized only in the recombinant carrying pfaB for EPA synthesis. Escherichia coli coexpressing a deleted construct comprising pfaA, pfaC, pfaD and pfaE for EPA and pfaB for DHA produced EPA and DHA. Both EPA and DHA were detected in bacteria that inherently contained pfa genes for DHA. These results suggest that PfaB is the key enzyme determining the final product in EPA or DHA biosynthesis.

引用

页码：170 / 176

页数：7

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