Chromatographic analysis of trans resveratrol in Italian wines: Comparisons between FL, UV and MS detection

被引：17

作者：

Buiarelli, F. ^{[1
]}

Coccioli, F. ^{[1
]}

Jasionowska, R. ^{[1
]}

Merolle, M. ^{[1
]}

Terracciano, A. ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

来源：

CHROMATOGRAPHIA | 2006年 / 64卷 / 7-8期

关键词：

column liquid chromatography; reversed phase LC; UV detection; fluorescence and MS detection; trans resveratrol in Italian wines;

D O I：

10.1365/s10337-006-0042-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Resveratrol (3,5,4'-trihydroxystilbene) is a phytoalexin that belongs to the group of stilbenes, known to occur in gropes and consequently in grape products. Its presence in wine is an important qualitative parameter because of the several beneficial effects on human health. The aim of this work is the development of a high-performance liquid chromatographic (HPLC) method for the determination of trans resveratrol in wines, and comparisons between the results obtained by different detection techniques: UV-vis spectroscopy, fluorescence spectroscopy and mass spectrometry. Resveratrol is analysed on a C-18 column using gradient elution. The method permits direct injection of sample, revealing to be time-saving, overcoming the need of sample pre-treatment steps. Detection limits were 154.8 ng mL(-1) by HPLC-UV, 118.0 ng mL(-1) by HPLC-FL and 48.0 ng mL(-1) by HPLC-MS. Trans resveratrol has been then quantified in a group of 52 wines derived from different Italian regions, cultivars and winemaking technologies by HPLC-UV.

引用

页码：475 / 481

页数：7

共 15 条

[1]

ASCIA KCR, 1995, CLIN CHIM ACTA, V235, P207

[2]

BENINCASA M, 1978, ANN CHIM, V77, P801

[3] Liquid chromatography-electrospray tandem mass spectrometry of cis-resveratrol and trans-resveratrol:: Development, validation, and application of the method to red wine, grape, and winemaking byproducts [J].

Careri, M ;

Corradini, C ;

Elviri, L ;

Nicoletti, I ;

Zagnoni, I .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (23) :6868-6874

[4] Direct HPLC analysis of quercetin and trans-resveratrol in red wine, grape, and winemaking byproducts [J].

Careri, M ;

Corradini, C ;

Elviri, L ;

Nicoletti, I ;

Zagnoni, I .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (18) :5226-5231

[5] Mass spectrometry in grape and wine chemistry. Part I: Polyphenols [J].

Flamini, R .

MASS SPECTROMETRY REVIEWS, 2003, 22 (04) :218-250

[6] INHIBITION OF HUMAN LDL OXIDATION BY RESVERATROL [J].

FRANKEL, EN ;

WATERHOUSE, AL ;

KINSELLA, JE .

LANCET, 1993, 341 (8852) :1103-1104

[7] ASSAY OF RESVERATROL GLUCOSIDES AND ISOMERS IN WINE BY DIRECT-INJECTION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

GOLDBERG, DM ;

NG, E ;

KARUMANCHIRI, A ;

YAN, J ;

DIAMANDIS, EP ;

SOLEAS, GJ .

JOURNAL OF CHROMATOGRAPHY A, 1995, 708 (01) :89-98

[8] PRODUCTION OF RESVERATROL BY VITIS-VINIFERA AND OTHER MEMBERS OF VITACEAE AS A RESPONSE TO INFECTION OR INJURY [J].

LANGCAKE, P ;

PRYCE, RJ .

PHYSIOLOGICAL PLANT PATHOLOGY, 1976, 9 (01) :77-86

[9]

LANGCAKE P, 1981, PHYSIOL PLANT PATHOL, V18, P213

[10] Optimisation of sample preparation for the determination of trans-resveratrol and other polyphenolic compounds in wines by high performance liquid chromatography [J].

Malovaná, S ;

Montelongo, FJG ;

Pérez, JP ;

Rodríguez-Delgado, MA .

ANALYTICA CHIMICA ACTA, 2001, 428 (02) :245-253

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