Determination of neomycin by LC-tandem mass spectrometry using hydrophilic interaction chromatography

被引：62

作者：

Oertel, R ^{[1
]}

Renner, U ^{[1
]}

Kirch, W ^{[1
]}

机构：

[1] Tech Univ Dresden, Inst Clin Pharmacol, Med Fac Carl Gustav Carus, D-01307 Dresden, Germany

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2004年 / 35卷 / 03期

关键词：

LC-MS-MS; hydrophilic interaction; HILIC; neomycin; aminoglycoside;

D O I：

10.1016/j.jpba.2004.01.018

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A specific, and automated method was developed to quantitate neomycin in human serum. Samples were prepared with an automated solid phase extraction (SPE). The hydrophilic interaction chromatography (HILIC) was used for additional sample cleanup and baseline separation. The analyte neomycin was detected with electrospray ionisation tandem mass spectrometry (ESI-MS-MS). Using a volume of 500 mul biological sample the lower limit of quantification was 100 ng/ml. The described HILIC-MS-MS method is suitable for clinical and pharmcokinetical investigations of neomycin. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：633 / 638

页数：6

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