Structural Elucidation of Novel Saponins in the Sea Cucumber Holothuria lessoni

被引:41
|
作者
Bahrami, Yadollah [1 ,2 ,3 ,4 ]
Zhang, Wei [1 ,2 ,3 ]
Chataway, Tim [5 ]
Franco, Chris [1 ,2 ,3 ]
机构
[1] Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Adelaide, SA 5042, Australia
[2] Flinders Univ S Australia, Ctr Marine Bioprod Dev, Adelaide, SA 5042, Australia
[3] Australian Seafood Cooperat Res Ctr, Adelaide, SA 5042, Australia
[4] Kermanshah Univ Med Sci, Med Biol Res Ctr, Kermanshah 6714415185, Iran
[5] Flinders Univ S Australia, Sch Med, Flinders Prote Facil, Adelaide, SA 5042, Australia
来源
MARINE DRUGS | 2014年 / 12卷 / 08期
关键词
sea cucumber viscera; saponins; bioactive compounds; MALDI; ESI; mass spectrometry; HPCPC; triterpene glycosides; structure elucidation; marine invertebrate; Echinodermata; holothurian; CYTOTOXIC TRITERPENE GLYCOSIDES; TANDEM MASS-SPECTROMETRY; MARINE NATURAL-PRODUCTS; BIOLOGICAL-ACTIVITIES; STAUROCUCUMIS LIOUVILLEI; RAPID IDENTIFICATION; PENTAMERA-CALCIGERA; PLANT-EXTRACTS; NORTH COAST; OLIGOGLYCOSIDES;
D O I
10.3390/md12084439
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Sea cucumbers are prolific producers of a wide range of bioactive compounds. This study aimed to purify and characterize one class of compound, the saponins, from the viscera of the Australian sea cucumber Holothuria lessoni. The saponins were obtained by ethanolic extraction of the viscera and enriched by a liquid-liquid partition process and adsorption column chromatography. A high performance centrifugal partition chromatography (HPCPC) was applied to the saponin-enriched mixture to obtain saponins with high purity. The resultant purified saponins were profiled using MALDI-MS/MS and ESI-MS/MS which revealed the structure of isomeric saponins to contain multiple aglycones and/or sugar residues. We have elucidated the structure of five novel saponins, Holothurins D/E and Holothurinosides X/Y/Z, along with seven reported triterpene glycosides, including sulfated and non-sulfated saponins containing a range of aglycones and sugar moieties, from the viscera of H. lessoni. The abundance of novel compounds from this species holds promise for biotechnological applications.
引用
收藏
页码:4439 / 4473
页数:35
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