BKCa channels regulate the immunomodulatory properties of WJ-MSCs by affecting the exosome protein profiles during the inflammatory response

被引：16

作者：

Song, Ahui ^{[1
]}

Wang, Jingjing ^{[1
]}

Tong, Yan ^{[1
]}

Fang, Junyan ^{[1
]}

Zhang, Yi ^{[3
]}

Zhang, Huiping ^{[3
]}

Ruan, Hongqiang ^{[3
]}

Wang, Kai ^{[2
]}

Liu, Yingli ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Nephrol, Sch Med, 639 Zhizaoju Rd, Shanghai, Peoples R China

[2] Tongji Univ, Sch Med, Clin & Translat Res Ctr, Shanghai Matern & Infant Hosp 1, Shanghai, Peoples R China

[3] Shanghai Appl Prot Technol Co Ltd, Ctr Res & Dev, 58 Yuanmei Rd, Shanghai, Peoples R China

来源：

STEM CELL RESEARCH & THERAPY | 2020年 / 11卷 / 01期

基金：

中国国家自然科学基金;

关键词：

BKCa channels; WJ-MSCs; Exosomes; Proteomics; Macrophages; MESENCHYMAL STROMAL CELLS; INDUCED ACTIVATION; GENE-EXPRESSION; ION CHANNELS; STEM-CELLS; MITOCHONDRIAL; PROLIFERATION; ROLES; POLARIZATION; MACROPHAGES;

D O I：

10.1186/s13287-020-01952-9

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

BackgroundWharton's jelly-derived mesenchymal stem cells (WJ-MSCs) from the human umbilical cord have been studied extensively due to their immunomodulatory functions. Large-conductance Ca2+-activated K+ (BKCa channels) channels are involved in many inflammatory responses, but their involvement in the anti-inflammatory activity of WJ-MSCs is unknown. The underlying molecular mechanism, through which BKCa channels mediate the immunomodulation of WJ-MSC, which may include changes in exosomes proteomics, has not yet been clarified.MethodsAlizarin staining, Oil Red O staining, and flow cytometry were used to identify WJ-MSCs, which were isolated from human umbilical cord Wharton's jelly. BKCa channels were detected in WJ-MSCs using western blotting, real-time polymerase chain reaction (real-time PCR), and electrophysiology, and cytokine expression was examined using real-time PCR and enzyme-linked immunosorbent assays (ELISAs). Exosomes were characterized using transmission electron microscopy and nanoparticle tracking analysis. Proteomics analysis was performed to explore exosomal proteomic profiles.ResultsThe cells derived from human umbilical cord Wharton's jelly were identified as MSCs. BKCa channels were detected in the isolated WJ-MSCs, and the expression of these channels increased after lipopolysaccharide (LPS) stimulation. BKCa channels blockade in LPS-treated WJ-MSCs induced apoptosis and decreased interleukin-6 (IL-6) expression. Furthermore, THP-1 cells (human monocytic cell line) stimulated with LPS/interferon gamma (IFN-gamma) produced more anti-inflammatory cytokines after treatment with exosomes derived from BKCa channel-knockdown WJ-MSCs (si-exo). We also observed altered expression of mitochondrial ATP synthase alpha subunit (ATP5A1), filamin B, and other proteins in si-exo, which might increase the anti-inflammatory activity of macrophages.ConclusionsOur study described the functional expression of BKCa channels in WJ-MSCs, and BKCa channels regulated the immunomodulatory properties of WJ-MSCs by affecting the exosomal protein profiles during the inflammatory response.

引用

页数：16

共 61 条

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