Mesenchymal stem cell-derived exosomes promote neurogenesis and cognitive function recovery in a mouse model of Alzheimer's disease

被引：171

作者：

Reza-Zaldivar, Edwin E. ^{[1
]}

Hernandez-Sapiens, Mercedes A. ^{[1
]}

Gutierrez-Mercado, Yanet K. ^{[1
]}

Sandoval-Avila, Sergio ^{[1
]}

Gomez-Pinedo, Ulises ^{[2
]}

Marquez-Aguirre, Ana L. ^{[1
]}

Vazquez-Mendez, Estefania ^{[1
]}

Padilla-Camberos, Eduardo ^{[1
]}

Canales-Aguirre, Alejandro A. ^{[1
]}

机构：

[1] Ctr Invest & Asistencia Tecnol & Diseno Estado Ja, Unidad Biotecnol Med & Farmaceut, Unidad Evaluac Preclin, Guadalajara, Jalisco, Mexico

[2] Hosp Clin San Carlos, IdISSC Hlth Res Inst, Dept Neurosurg & Neurol, Regenerat Med Unit,Neurosci Inst, Madrid, Spain

来源：

NEURAL REGENERATION RESEARCH | 2019年 / 14卷 / 09期

关键词：

Alzheimer's disease; neurodegenerative disease; cognitive impairment; memory; Alzheimer's disease mouse model; mesenchymal stem cell; exosomes; neurogenesis; cognitive improvement; cell-free therapy; neural regeneration; LONG-TERM-MEMORY; OXIDATIVE STRESS; RAT MODEL; TRANSPLANTATION; PATHOLOGY; TOXICITY; NEURONS; STROKE; INDUCE; PHASE;

D O I：

10.4103/1673-5374.255978

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Studies have shown that mesenchymal stem cell-derived exosomes can enhance neural plasticity and improve cognitive impairment. The purpose of this study was to investigate the effects of mesenchymal stem cell-derived exosomes on neurogenesis and cognitive capacity in a mouse model of Alzheimer's disease. Alzheimer's disease mouse models were established by injection of beta amyloid 1-42 aggregates into dentate gyrus bilaterally. Morris water maze and novel object recognition tests were performed to evaluate mouse cognitive deficits at 14 and 28 days after administration. Afterwards, neurogenesis in the subventricular zone was determined by immunofluorescence using doublecortin and PSA-NCAM antibodies. Results showed that mesenchymal stem cells-derived exosomes stimulated neurogenesis in the subventricular zone and alleviated beta amyloid 1-42-induced cognitive impairment, and these effects are similar to those shown in the mesenchymal stem cells. These findings provide evidence to validate the possibility of developing cell-free therapeutic strategies for Alzheimer's disease. All procedures and experiments were approved by Institutional Animal Care and Use Committee (CICUAL) (approval No. CICUAL 2016-011) on April 25, 2016.

引用

页码：1626 / 1634

页数：9

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