Utility of integrated HIV-1 DNA quantification in cure studies

被引：4

作者：

Ruggiero, Alessandra ^{[1
]}

Malatinkova, Eva ^{[2
]}

Rutsaert, Sofie ^{[2
]}

Paxton, William A. ^{[1
]}

Vandekerckhove, Linos ^{[2
]}

De Spiegelaere, Ward ^{[3
]}

机构：

[1] Univ Liverpool, Inst Infect & Global Hlth, Dept Clin Infect Microbiol & Immunol CIMI, Liverpool, Merseyside, England

[2] Univ Ghent, Fac Med & Hlth Sci, Dept Internal Med, HIV Cure Res Ctr, Ghent, Belgium

[3] Univ Ghent, Fac Vet Sci, Dept Morphol, Ghent, Belgium

来源：

FUTURE VIROLOGY | 2017年 / 12卷 / 04期

基金：

比利时弗兰德研究基金会;

关键词：

Alu-gag PCR; HIV-1; reservoir; integrated HIV-1 DNA; replication competency; HISTONE DEACETYLASE INHIBITORS; CD4(+) T-CELLS; SUPPRESSIVE ANTIRETROVIRAL THERAPY; LATENT RESERVOIR; PERIPHERAL-BLOOD; RALTEGRAVIR INTENSIFICATION; ONGOING REPLICATION; DISEASE PROGRESSION; TYPE-1; REPLICATION; VIRAL PERSISTENCE;

D O I：

10.2217/fvl-2016-0130

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Numerous HIV-1 curative strategies have been proposed to eradicate the virus reservoir pool that remains integrated within target cells, despite successful antiretroviral therapy. To test the impact of such interventions on this reservoir, a universal marker of persistence is needed. Quantifying integrated HIV-1 DNA load has been proposed as a strong virological marker. In this paper, we provide a detailed description of the most commonly used assays to quantify integrated HIV-1 DNA and applications in relevant clinical studies produced over the last 20 years with a major focus on the recent literature. We discuss the potential for using this marker of virological persistence and the technical limitations that need to be addressed.

引用

页码：215 / 225

页数：11

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