The effect of sodium hyaluronate on the growth of rabbit cornea epithelial cells in vitro

被引:18
作者
Miyazaki, T
Miyauchi, S
Nakamura, T
Takeshita, S
Horie, K
机构
[1] Seikagaku Corporation, Tokyo Research Institute, Higashiyamato, Tokyo 207, 3-1253, Tateno
关键词
D O I
10.1089/jop.1996.12.409
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
The effect of sodium hyaluronate (Na-HA) on the growth of rabbit corneal epithelial cells was investigated in vine by using MTT assay and [H-3]-thymidine incorporation. Na-HA, with a molecular weight of 86 x 10(4), enhanced the growth of the corneal epithelial cells cultured on a fibronectin (FN) coated dish, but when cells were cultured on a noncoated dish, Na-HA did not enhance the growth of the cells. Na-HA, labeled with 5-aminofluorescein (FA-HA), bound to the corneal epithelial cells, and the CD44-like molecule was identified on the cells by using a monoclonal antibody, OS/37. Furthermore, the binding of FA-HA to the cells was significantly inhibited by the monoclonal antibody. These results suggest that Na-HA binds to the CD44-like molecule associated with FN and then enhances the growth of the corneal epithelial cells.
引用
收藏
页码:409 / 415
页数:7
相关论文
共 24 条
[1]   THE HYALURONATE RECEPTOR IS PREFERENTIALLY EXPRESSED ON PROLIFERATING EPITHELIAL-CELLS [J].
ALHO, AM ;
UNDERHILL, CB .
JOURNAL OF CELL BIOLOGY, 1989, 108 (04) :1557-1565
[2]   CD44 IS THE PRINCIPAL CELL-SURFACE RECEPTOR FOR HYALURONATE [J].
ARUFFO, A ;
STAMENKOVIC, I ;
MELNICK, M ;
UNDERHILL, CB ;
SEED, B .
CELL, 1990, 61 (07) :1303-1313
[3]   LOCALIZATION OF HYALURONIC-ACID, CHONDROITIN SULFATE, AND CD44 IN RABBIT CORNEA [J].
ASARI, A ;
MIYAUCHI, S ;
TAKAHASHI, T ;
KOHNO, K ;
UCHIYAMA, Y .
ARCHIVES OF HISTOLOGY AND CYTOLOGY, 1992, 55 (05) :503-511
[4]  
BOURGUIGNON LYW, 1993, J IMMUNOL, V151, P6634
[5]   THE HYALURONAN RECEPTOR (CD44) PARTICIPATES IN THE UPTAKE AND DEGRADATION OF HYALURONAN [J].
CULTY, M ;
NGUYEN, HA ;
UNDERHILL, CB .
JOURNAL OF CELL BIOLOGY, 1992, 116 (04) :1055-1062
[6]  
DOILLON CJ, 1987, BIOMATERIALS, V8, P195, DOI 10.1016/0142-9612(87)90063-9
[7]  
FITZSIMMONS TD, 1992, INVEST OPHTH VIS SCI, V33, P3011
[8]  
INOUE M, 1993, INVEST OPHTH VIS SCI, V34, P2313
[9]   LYMPHOCYTE CD44 BINDS THE COOH-TERMINAL HEPARIN-BINDING DOMAIN OF FIBRONECTIN [J].
JALKANEN, S ;
JALKANEN, M .
JOURNAL OF CELL BIOLOGY, 1992, 116 (03) :817-825
[10]   INVITRO GROWTH OF ADULT AMPHIBIAN (XENOPUS-LAEVIS) HEPATOCYTES AND CHARACTERIZATION OF HEPATOCYTE-PROLIFERATING ACTIVITY IN HOMOLOGOUS SERUM [J].
KAWAHARA, A ;
ISHIKAWA, S ;
AMANO, M .
EXPERIMENTAL CELL RESEARCH, 1985, 159 (02) :344-352