The synthesis of peptides and proteins containing non-natural amino acids

被引：123

作者：

Hodgson, DRW ^{[1
]}

Sanderson, JM ^{[1
]}

机构：

[1] Univ Durham, Dept Chem, Durham DH1 3LE, England

来源：

CHEMICAL SOCIETY REVIEWS | 2004年 / 33卷 / 07期

关键词：

D O I：

10.1039/b312953p

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Methods for the incorporation of non-natural amino acids into proteins have advanced significantly over recent years and in this tutorial review we aim to give a general overview of the area. These techniques offer the possibility of modulating the structures and functions of proteins and thus permit the generation of novel designed systems for both biocatalytic and mechanistic studies. Four complementary approaches are discussed in detail along with examples of their application. The advantages and disadvantages of each technique are also discussed.

引用

页码：422 / 430

页数：9

共 54 条

[31] Modular peptide synthetases involved in nonribosomal peptide synthesis [J].

Marahiel, MA ;

Stachelhaus, T ;

Mootz, HD .

CHEMICAL REVIEWS, 1997, 97 (07) :2651-2673

[32] The tyrocidine biosynthesis operon of Bacillus brevis: Complete nucleotide sequence and biochemical characterization of functional internal adenylation domains [J].

Mootz, HD ;

Marahiel, MA .

JOURNAL OF BACTERIOLOGY, 1997, 179 (21) :6843-6850

[33] Conditional protein splicing: A new tool to control protein structure and function in vitro and in vivo [J].

Mootz, HD ;

Blum, ES ;

Tyszkiewicz, AB ;

Muir, TW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (35) :10561-10569

[34] Using a solid-phase ribozyme aminoacylation system to reprogram the genetic code [J].

Murakami, H ;

Kourouklis, D ;

Suga, H .

CHEMISTRY & BIOLOGY, 2003, 10 (11) :1077-1084

[35] Facile aminoacylation of pdCpA dinucleotide with a nonnatural amino acid in cationic micelle [J].

Ninomiya, K ;

Kurita, T ;

Hohsaka, T ;

Sisido, M .

CHEMICAL COMMUNICATIONS, 2003, (17) :2242-2243

[36] A perturbed pKa at the binding site of the nicotinic acetylcholine receptor:: Implications for nicotine binding [J].

Petersson, EJ ;

Choi, A ;

Dahan, DS ;

Lester, HA ;

Dougherty, DA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (43) :12662-12663

[37] Generation of new enzymes via covalent modification of existing proteins [J].

Qi, DF ;

Tann, CM ;

Haring, D ;

Distefano, MD .

CHEMICAL REVIEWS, 2001, 101 (10) :3081-3111

[38] A GENERAL AND EFFICIENT ROUTE FOR CHEMICAL AMINOACYLATION OF TRANSFER-RNAS [J].

ROBERTSON, SA ;

ELLMAN, JA ;

SCHULTZ, PG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (07) :2722-2729

[39] An efficient system for the evolution of aminoacyl-tRNA synthetase specificity [J].

Santoro, SW ;

Wang, L ;

Herberich, B ;

King, DS ;

Schultz, PG .

NATURE BIOTECHNOLOGY, 2002, 20 (10) :1044-1048

[40] Utilization of alternate substrates by the first three modules of the epothilone synthetase assembly line [J].

Schneider, TL ;

Walsh, CT ;

O'Connor, SE .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (38) :11272-11273

← 1 2 3 4 5 6 →