Construction of a Robust Entropy-Driven DNA Nanomachine for Single-Molecule Detection of Rare Cancer Cells

被引：78

作者：

Ma, Fei ^{[1
]}

Wei, Shu-hua ^{[1
]}

Zhang, Chun-yang ^{[1
]}

机构：

[1] Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Shandong Prov Key Lab Clean Prod Fine Chem, Coll Chem Chem Engn & Mat Sci,Minist Educ,Key Lab, Jinan 250014, Shandong, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2019年 / 91卷 / 12期

基金：

中国国家自然科学基金;

关键词：

CIRCULATING TUMOR-CELLS; APTAMER-CONJUGATED NANOPARTICLES; PERIPHERAL-BLOOD; BREAST-CANCER; COLLECTION; PROBES;

D O I：

10.1021/acs.analchem.9b01617

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Accurate and sensitive detection of rare cancer cells such as circulating tumor cells (CTCs) has attracted great interest in the fields of clinical diagnosis and cancer research. However, the reported methods for cancer cell detection often involve a complicated platform and laborious procedures with a limited sensitivity. Herein, we construct a new entropy-driven DNA nanomachine for single-molecule detection of rare cancer cells. This assay employs the entropy-driven DNA nanomachine for efficient cancer cell recognition and signal amplification without the involvement of any expensive and unstable antibodies and enzymes, and it enables one-step detection of living cancer cells with high sensitivity and good specificity. This DNA nanomachine can be further applied for rare CTC detection in whole blood samples.

引用

页码：7505 / 7509

页数：5

共 38 条

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