Immunolocalization of dinitrogenase reductase produced by Klebsiella pneumoniae in association with Zea mays L.

被引：127

作者：

Chelius, MK

Triplett, EW ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA

[2] Univ Wisconsin, Brock Inst Environm Microbiol, Ctr Study Nitrogen Fixat, Madison, WI 53706 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 02期

关键词：

D O I：

10.1128/AEM.66.2.783-787.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The endophytic lifestyle of Klebsiella pneumoniae is described, including the production of dinitrogenase reductase by bacteria residing in maize root tissue. The green fluorescent protein (GFP) was used to detect the colonization of maize by K. pneumoniae strains 2028 and 342, These strains were found to reside in intercortical layers of the stem and within the region of maturation in the root. The production of dinitrogenase reductase by GFP-tagged bacteria was visualized using immunolocalization, This activity was only apparent when bacteria were supplied with an exogenous carbon source, The results suggest that maize provides a suitable habitat for K, pneumoniae and that this species is capable of producing nitrogenase under the appropriate plant cultivation conditions.

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页码：783 / 787

页数：5

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