PIBF positive uterine NK cells in the mouse decidua

被引:19
|
作者
Bogdan, Agnes [1 ,3 ,4 ]
Berta, Gergely [1 ,2 ]
Szekeres-Bartho, Julia [1 ,3 ,4 ]
机构
[1] Sch Med, Dept Med Biol, Pecs, Hungary
[2] MTA PTE Human Reprod Res Grp, Pecs, Hungary
[3] Univ Pecs, Janos Szentagothai Res Ctr, Pecs, Hungary
[4] Ctr Excellence, Endocrine Studies, Pecs, Hungary
关键词
Uterine NK cells; PIBF; Perforin; NATURAL-KILLER-CELLS; INDUCED BLOCKING FACTOR; PERFORIN-EXPRESSING LYMPHOCYTES; SPONTANEOUS-ABORTION; PROGESTERONE; PREGNANCY; DIFFERENTIATION; RECEPTOR; CLONING; UTERUS;
D O I
10.1016/j.jri.2016.12.001
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Though uterine NK cells (u NK cells) contain cytotoxic granules, and selectively over- express the genes of perforin and granzymes, during normal pregnancy, they are not cytotoxic. Progesterone is indispensable for the establishment and maintenance of pregnancy both in humans and in mice. Mouse uterine NK cells do not express the classical progesterone receptor, yet progesterone affects the recruitment and function of uterine NK cells, the latter partly via the Progesterone-Induced Blocking Factor (PIBF). We demonstrated PIBF positive granulated cells in the mouse decidua. The aim of this study was to characterize these cells by lectin immunohistochemistry and anti-perforin reactivity. PIBF+ granulated cells were absent from the deciduae of alymphoid mice, but appeared in the decidua of those that had been reconstituted with bone marrow from male BALB/c mice. PIBF+ granulated cells bound the DBA lectin, suggesting their NK cell nature, and also contained perforin, which co-localized with PIBF in the cytoplasmic granules. In anti-progesterone treated mice all of the PIBF+ cells were perforin positive at g. d. 12.5, in contrast to the 54% perforin positivity of PIBF+ cells in untreated mice. Conclusion: The PIBF+ granulated cells in the decidua belong to the NK population, and PIPS co-localizes with perforin in the cytoplasmic granules. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:38 / 43
页数:6
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