MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience

被引：157

作者：

Collot, Mayeul ^{[1
]}

Ashokkumar, Pichandi ^{[1
]}

Anton, Halina ^{[1
]}

Boutant, Emmanuel ^{[1
]}

Faklaris, Orestis ^{[2
]}

Galli, Thierry ^{[2
,3
]}

Mely, Yves ^{[1
]}

Danglot, Lydia ^{[2
,3
]}

Klymchenko, Andrey S. ^{[1
]}

机构：

[1] Univ Strasbourg, Fac Pharm, Lab Biophoton & Pathol, UMR CNRS 7021, 74 Route Rhin, F-67401 Illkirch Graffenstaden, France

[2] Univ Paris Diderot, Sorbonne Paris Cite, Inst Jacques Monod, CNRS UMR 7592, F-75013 Paris, France

[3] Univ Paris 05, Membrane Traff Hlth & Diseased Brain, IPNP, INSERM,U1266, 102 Rue Sante, F-75014 Paris, France

来源：

CELL CHEMICAL BIOLOGY | 2019年 / 26卷 / 04期

基金：

欧洲研究理事会;

关键词：

OPTICAL RECONSTRUCTION MICROSCOPY; PLASMA-MEMBRANES; FLUOROGENIC PROBES; LIPID RAFTS; RED; CELLS; CHOLESTEROL; DIFFUSION; TRACKING; NEURONS;

D O I：

10.1016/j.chembiol.2019.01.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The proper staining of the plasma membrane (PM) is critical in bioimaging as it delimits the cell. Herein, we developed MemBright, a family of six cyanine-based fluorescent turn-on PM probes that emit from orange to near infrared when reaching the PM, and enable homogeneous and selective PM staining with excellent contrast in mono- and two-photon microscopy. These probes are compatible with long-term live-cell imaging and immunostaining. Moreover, MemBright label neurons in a brighter manner than surrounding cells, allowing identification of neurons in acute brain tissue sections and neuromuscular junctions without any use of transfection or transgenic animals. In addition, MemBright probes were used in super-resolution imaging to unravel the neck of dendritic spines. 3D multicolor dSTORM in combination with immunostaining revealed en-passant synapse displaying endogenous glutamate receptors clustered at the axonal-dendritic contact site. MemBright probes thus constitute a universal toolkit for cell biology and neuroscience biomembrane imaging with a variety of microscopy techniques.

引用

页码：600 / +

页数：22

共 77 条

[1] Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm [J].