Serial Block-Face Imaging and its Potential for Reconstructing Diminutive Cell Systems: A Case Study from Arthropods

被引：7
作者：
Lipke, Elisabeth [1 ]
Hoernschemeyer, Thomas [2 ]
Pakzad, Anahita [3 ]
Booth, Christopher R. [3 ]
Michalik, Peter [1 ,4 ]
机构：
[1] Ernst Moritz Arndt Univ Greifswald, Zool Inst & Museum, D-17487 Greifswald, Germany
[2] Univ Gottingen, Johann Friedrich Blumenbach Inst Zool & Anthropol, Dept Morphol Systemat & Evolutionary Biol, D-37073 Gottingen, Germany
[3] Gatan Inc, Pleasanton, CA USA
[4] Amer Museum Nat Hist, Div Invertebrate Zool, New York, NY 10024 USA
来源：
MICROSCOPY AND MICROANALYSIS | 2014年 / 20卷 / 03期
关键词：
SBFSEM; ssTEM; enhanced heavy metal staining; artifact; spider sperm; 3-DIMENSIONAL RECONSTRUCTION; ELECTRON-MICROSCOPY; SECTION THICKNESS; TISSUE; ULTRASTRUCTURE; SEGMENTATION; MORPHOLOGY; SYNAPSES; CIRCUIT;
D O I：
10.1017/S1431927614000087
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Until recently, three-dimensional reconstruction on an ultrastructural level was only possible using serial section transmission electron microscopy (ssTEM). However, ssTEM is highly challenging and prone to artifacts as, e.g., section loss and image distortions. New methods, such as serial block-face scanning electron microscopy (SBFSEM) overcome these limitations and promise a high lateral resolution. However, little is known about the usability of SBFSEM in diminutive, but highly complex cellular systems. We used spider sperm (similar to 3 mu m in diameter), which fulfills these conditions, to analyze the potential of SBFSEM compared with ssTEM. Our data suggest that the resolution obtained by SBFSEM allows depicting structures on a cellular level and is sufficient to discriminate subcellular components, but is highly dependent on previous staining procedures and electron density of the target structures.
引用
收藏
页码：946 / 955
页数：10
相关论文
共 56 条
  • [1] Andersson-Cedergren E., 1959, J. Ultrastruct. Res, V2, P5, DOI [DOI 10.1016/S0889-1605(59)80002-1, 10.1016/S0889-1605(59)80002-1]
  • [2] Andres B, 2008, LECT NOTES COMPUT SC, V5096, P142, DOI 10.1007/978-3-540-69321-5_15
  • [3] 3D segmentation of SBFSEM images of neuropil by a graphical model over supervoxel boundaries
    Andres, Bjoern
    Koethe, Ullrich
    Kroeger, Thorben
    Helmstaedter, Moritz
    Briggman, Kevin L.
    Denk, Winfried
    Hamprecht, Fred A.
    [J]. MEDICAL IMAGE ANALYSIS, 2012, 16 (04) : 796 - 805
  • [4] [Anonymous], 2010, MICROSCOPY-JPN
  • [5] Ultrastructure of dendritic spines: correlation between synaptic and spine morphologies
    Arellano, Jon I.
    Benavides-Piccione, Ruth
    DeFelipe, Javier
    Yuste, Rafael
    [J]. FRONTIERS IN NEUROSCIENCE, 2007, 1 (01): : 131 - 143
  • [6] Imaging Transient Blood Vessel Fusion Events in Zebrafish by Correlative Volume Electron Microscopy
    Armer, Hannah E. J.
    Mariggi, Giovanni
    Png, Ken M. Y.
    Genoud, Christel
    Monteith, Alexander G.
    Bushby, Andrew J.
    Gerhardt, Holger
    Collinson, Lucy M.
    [J]. PLOS ONE, 2009, 4 (11):
  • [7] Towards neural circuit reconstruction with volume electron microscopy techniques
    Briggman, Kevin L.
    Denk, Winfried
    [J]. CURRENT OPINION IN NEUROBIOLOGY, 2006, 16 (05) : 562 - 570
  • [8] Wiring specificity in the direction-selectivity circuit of the retina
    Briggman, Kevin L.
    Helmstaedter, Moritz
    Denk, Winfried
    [J]. NATURE, 2011, 471 (7337) : 183 - U67
  • [9] Imaging three-dimensional tissue architectures by focused ion beam scanning electron microscopy
    Bushby, Andrew J.
    P'ng, Kenneth M. Y.
    Young, Robert D.
    Pinali, Christian
    Knupp, Carlo
    Quantock, Andrew J.
    [J]. NATURE PROTOCOLS, 2011, 6 (06) : 845 - 858
  • [10] DEGROOT DMG, 1988, J MICROSC-OXFORD, V151, P23
123456