Overcoming the trade-off between grain weight and number in wheat by the ectopic expression of expansin in developing seeds leads to increased yield potential

被引:102
作者
Calderini, Daniel F. [1 ]
Castillo, Francisca M. [1 ,2 ]
Arenas-M, Anita [1 ,2 ]
Molero, Gemma [3 ]
Reynolds, Matthew P. [3 ]
Craze, Melanie [4 ]
Bowden, Sarah [4 ]
Milner, Matthew J. [4 ]
Wallington, Emma J. [4 ]
Dowle, Adam [5 ]
Gomez, Leonardo D. [5 ]
McQueen-Mason, Simon J. [5 ]
机构
[1] Univ Austral Chile, Inst Plant Prod & Protect, Campus Isla Teja, Valdivia 5090000, Chile
[2] Univ Austral Chile, Fac Sci, Inst Biochem & Microbiol, Valdivia 5090000, Chile
[3] Int Maize & Wheat Improvement Ctr CIMMYT, Texcoco 56237, Mexico
[4] NIAB, 93 Lawrence Weaver Rd, Cambridge CB3 0LE, England
[5] Univ York, Biol Dept, CNAP, Wentworth Way, York YO10 5YW, N Yorkshire, England
基金
英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;
关键词
expansin protein; grain number; grain weight; grasses; pericarp; trade‐ off; transgenic; KERNEL WEIGHT; GROWTH; PREANTHESIS; PROTEINS; TRANSFORMATION; TEMPERATURE; EFFICIENCY; DYNAMICS; SPIKE; SIZE;
D O I
10.1111/nph.17048
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Wheat is the most widely grown crop globally, providing 20% of all human calories and protein. Achieving step changes in genetic yield potential is crucial to ensure food security, but efforts are thwarted by an apparent trade-off between grain size and number. Expansins are proteins that play important roles in plant growth by enhancing stress relaxation in the cell wall, which constrains cell expansion. Here, we describe how targeted overexpression of an alpha-expansin in early developing wheat seeds leads to a significant increase in grain size without a negative effect on grain number, resulting in a yield boost under field conditions. The best-performing transgenic line yielded 12.3% higher average grain weight than the control, and this translated to an increase in grain yield of 11.3% in field experiments using an agronomically appropriate plant density. This targeted transgenic approach provides an opportunity to overcome a common bottleneck to yield improvement across many crops.
引用
收藏
页码:629 / 640
页数:12
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