Differential Expression Profile of Long Non-coding RNAs during Differentiation of Cardiomyocytes

被引：16

作者：

Zhu, Shasha ^{[1
]}

Hu, Xiaoshan ^{[1
]}

Han, Shuping ^{[1
]}

Yu, Zhangbin ^{[1
]}

Peng, Yuzhu ^{[1
]}

Zhu, Jingai ^{[1
]}

Liu, Xuehua ^{[1
]}

Qian, Lingmei ^{[2
]}

Zhu, Chun ^{[1
]}

Li, Mengmeng ^{[1
]}

Song, Guixian ^{[2
]}

Guo, Xirong ^{[1
]}

机构：

[1] Nanjing Med Univ, Nanjing Matern & Child Hlth Care Hosp, Dept Pediat, State key Lab Reprod Med, Nanjing 210029, Jiangsu, Peoples R China

[2] Nanjing Med Univ, Affiliated Hosp 1, Dept Cardiol, Nanjing 210029, Jiangsu, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MEDICAL SCIENCES | 2014年 / 11卷 / 05期

基金：

中国国家自然科学基金;

关键词：

lncRNAs; differentiation; caridiomyocytes; microarrays; EMBRYONAL CARCINOMA-CELLS; P19; CELLS; HEART; GENE; METASTASIS; MICRORNAS; COMPLEXES; MECHANISM; PROMOTES; CANCER;

D O I：

10.7150/ijms.7849

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Many long non-coding RNAs (lncRNAs) are species specific and seem to be less conserved than protein-coding genes. Some of them are involved in the development of the lateral mesoderm in the heart and in the differentiation of cardiomyocytes. The purpose of the study was to investigate the expression profiles of lncRNAs during the differentiation of P19 cells into cardiomyocytes, with a view to studying the biological function of lncRNAs and their involvement in the mechanism of heart development. First, we observed the morphology of P19 cells during differentiation using an inverted microscope. Then, cardiac troponin T (cTnT) expression was detected to validate that the cells had successfully differentiated into cardiac myocytes by real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR) and western blotting. Lastly, the expression profile of lncRNA genes was obtained using an lncRNA microarray and real-time RT-PCR analyses. The microarray results showed that 40 lncRNAs were differentially expressed, of which 28 were upregulated and 12 were downregulated in differentiated cardiomyocytes. The differentially expressed lncRNAs were further validated. Our results illustrated a critical role of lncRNAs during the differentiation of P19 cells into cardiac myocytes, which will provide the foundation for further study of the biological functions of lncRNAs and the mechanism of heart development.

引用

页码：500 / 507

页数：8

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